STEREOCHEMISTRY OF ENZYME REACTIONS

酶反应的立体化学

• 批准号: 3281074
• 负责人: HEINZ G FLOSS
• 金额: $ 26.06万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3281074
• 关键词: 1 carbon compound; Methanobacteriaceae; alkaloids; aminoacid biosynthesis; chemical cleavage; chemical structure function; enzyme mechanism; enzyme structure; isoquinolines; molecular rearrangement; nuclear magnetic resonance spectroscopy; stereochemistry; transmethylation

In the continuation of our studies on the stereochemistry of enzyme reactions, the main emphasis will continue to be on the steric course of reactions of one-carbon metabolism. Stereochemical questions to be addressed relate to methylenedioxy bridge formation in benzylisoquinoline alkaloids, tryptophan methylation at C-2 of indole, the methylenetetrahydrofolate reductase reaction, B12-containing enzymes involved in methyl transfers, methane formation in methanogenic bacteria and methane oxidation by methane oxygenase, and C-P bond cleavage in the degradation of ethylphosphonate. In addition, two projects initiated under other grants will be incorporated into this program. One of these deals with the stereochemical analysis of polyketide formation from chiral malonate and/or chiral acetate using purified enzymes (chalcone formation) or whole cells (some fungal metabolites). The other is the stereochemical analysis of the unique rearrangement reaction by which ribulose 5-phosphate is converted into the novel sugar, 1-deoxytetrulose 4-phosphate, a precursor of riboflavin.

在酶的立体化学研究中， 反应，主要重点将继续放在立体过程中， 一碳代谢的反应。 立体化学问题 涉及苄基异喹啉中亚甲二氧基桥形成 生物碱，吲哚C-2位色氨酸甲基化， 亚甲基四氢叶酸还原酶反应，含B12酶 参与甲基转移，产甲烷细菌中的甲烷形成 和甲烷氧化的甲烷加氧酶，和C-P键断裂在 乙基膦酸酯的降解。 此外，还启动了两个项目 其他赠款将纳入该方案。 其中一笔交易 通过对手性聚酮形成的立体化学分析， 丙二酸酯和/或手性乙酸酯（查耳酮形成） 或整个细胞（一些真菌代谢物）。 另一种是立体化学 5-磷酸核酮糖独特重排反应的分析 转化为新的糖，1-脱氧四果糖4-磷酸， 核黄素的前体。