NMR STUDIES OF THE MECHANISMS OF ACTION OF TRP-REPRESSOR

TRP 阻遏物作用机制的 NMR 研究

• 批准号: 3283080
• 负责人: OLEG JARDETZKY
• 金额: $ 16.07万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1993-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3283080
• 关键词: DNA; DNA binding protein; allosteric site; chemical binding; chemical structure; conformation; fluorimetry; genetic operator element; genetic transcription; nuclear magnetic resonance spectroscopy; nucleic acid sequence; protein structure; relaxation spectrometry; thermodynamics; transcription factor; tryptophan

The long term objectives are to understand at the molecular level the recognition of specific sequences of DNA by repressors, and the allosteric modulation of this recognition process by metabolites. The specific aims are to investigate the thermodynamics and mechanism of the interaction of the trp repressor with its operator DNA and other, non-operator DNA sequences, in the presence and absence of specific modulatory molecules (tryptophan and desaminotryptophan). The repressor protein and the DNA will also be extensively characterized, both structurally and functionally, using advanced high resolution NMR methods, particularly two dimensional techniques and measurements of relaxation times. Once the components have been fully characterized, the changes that occur on forming various complexes will be compared, and detailed information regarding the molecular basis for such recognition will be available. These recognition processes are an integral part of the control of gene expression a topic which lies at the heart of modern molecular biology and relates indirectly to the development of suppression of certain kinds of cancer.

长期目标是在分子水平上理解 阻遏物对DNA特定序列的识别，以及 这种识别过程的变构调节， 代谢物。 具体目的是调查 trp相互作用的热力学和机理 阻遏物及其操纵基因DNA和其他非操纵基因DNA 序列，在存在和不存在特异性调节剂的情况下， 分子（色氨酸和脱氨基色氨酸）。 阻遏物 蛋白质和DNA也将得到广泛的表征， 结构和功能，使用先进的高分辨率NMR 方法，特别是二维技术和测量 放松时间。 一旦组件完全 其特征在于，在形成各种复合物时发生的变化 将进行比较，并详细介绍有关分子 这样的认可将有依据。 这些识别 过程是基因表达控制的一个组成部分 这是现代分子生物学的核心课题， 间接关系到发展抑制某些 各种癌症。