STEREOCHEMISTRY OF ENZYME REACTIONS

在酶的立体化学研究中， 反应的主要重点将是对各种反应的立体过程 一碳代谢和脱氧己糖形成的机制， 目前仍在努力完成正在进行的关于下列问题的研究： 磷酸吡哆醛酶和色氨酸的异戊二烯化， 锁骨 对一碳代谢的研究将考察 DNA甲基化的立体化学方面，蛋氨酸生物合成， 形成亚甲二氧基官能团和小檗碱桥， 生物碱的生物合成，以及与硫霉素有关的双重甲基化 生物合成 在脱氧己糖形成的工作中，我们将研究 立体化学和机械方面的形成 通过吡哆胺从CDP-4-酮基-6-脱氧葡萄糖得到CDP-3，6-二脱氧葡萄糖 沙门氏菌的磷酸盐依赖酶 探索性研究还将 在链霉菌中进行2，6-二脱氧己糖的生物合成 以及焦磷酸硫胺素催化酶的立体化学 反应.

In the continuation of our studies on the stereochemistry of enzyme reactions the major focus will be on the steric course of various reactions of one-carbon metabolism and on the mechanism of deoxyhexose formation, with some effort still devoted to the completion of ongoing studies on pyridoxal phosphate enzymes and on the isoprenylation of tryptophan in Claviceps. The studies on one-carbon metabolism will examine stereochemical aspects of DNA methylation, methionine biosynthesis, the formation of the methylenedioxy function and the berberine bridge in alkaloid biosynthesis, and the double methylation involved in thienamycin biosynthesis. In the work on deoxyhexose formation we will study stereochemical and mechanistic aspects of the formation of CDP-3,6-dideoxyglucose from CDP-4-keto-6-deoxyglucose by a pyridoxamine phosphate-dependent enzyme from Salmonella. Exploratory studies will also be carried out on the biosynthesis of 2,6-dideoxyhexoses in Streptomyces and on the stereochemistry of thiamine pyrophosphate-catalyzed enzyme reactions.