ELECTROPHORESIS OF HUMAN MALIGNANT HYPERTHERMIC MUSCLE

恶性高热（MH）是一种药物遗传性疾病， 挥发性麻醉剂和非去极化肌肉松弛剂。 其发病率 是1/15，000到1/50，000的麻醉剂， 大约30%。 MH综合征的特征是代谢性酸中毒， 心动过速肌肉僵硬体温明显升高 的 MH的术前诊断是困难的，因为它需要大量的 肌肉活检，实验室和生理学家致力于MH诊断 问题研究 那些有家族史的人可以进行筛查 肌肉活检检测MH，但可用的肌肉检测也携带 相当程度的不确定性。 这项建议旨在扩大我们的 以前的观察表明，存在新的低分子量蛋白质 在肌肉中。 我们打算获取更多的肌肉样本 确诊为精神分裂症的患者。 肌肉蛋白质将 分离并鉴定额外的蛋白质。 的 额外的MH蛋白质将通过光密度扫描定量， 电泳凝胶和MH易感性与 制造的额外MH蛋白质的量。

Malignant hyperthermia (MH) is a pharmacogenetic disorder provoked by volatile anesthetics and non-depolarizing muscle relaxants. Its incidence is between 1 in 15,000 and 1 in 50,000 anesthetics with a mortality of approximately 30%. The MH syndrome is characterized by metabolic acidosis, tachycardia, muscle rigidity and markedly elevated temperature. The preoperative diagnosis of MH is difficult because it requires a large muscle biopsy, a laboratory and physiologist dedicated to MH diagnostic studies. Those individuals with a previous family history can be screened for MH by muscle biopsy but the available tests of the muscle also carry a fair degree of uncertainty. This proposal is intended to extend our previous observations that novel low molecular weight proteins are present in MH muscle. We intend to obtain a greater number of muscle specimens from patients who have had verified MH episodes. The muscle proteins will be electrophoresed and the extra proteins isolated and characterized. The extra MH proteins will be quantitated by densitometric scans of the electrophoretic gels and a correlation between MH susceptibility and the amount of extra MH proteins made.