ANESTHETIC DEPRESSION OF THE MYOCARDIUM

在麻醉的患者中观察到了低血压和心脏骤停。 麻醉剂，氟烷，安氟醚和硫喷妥钠。这些 如今麻醉药在临床上得到了广泛的应用。初步证据表明 这些麻醉剂可能会减少人体所需的钙 心脏收缩。这个项目的目的是研究 以上提到的麻醉剂对心肌细胞结构的影响 调节钙流，控制收缩强度。元素 那将被研究的是：肌膜(SL)，外膜的 肌肉细胞；肌浆网(SR)，一种膜网络， 控制肌钙和肌钙蛋白(TN)的量，肌钙蛋白是一种 通过改变其形状和启动钙离子结合来响应钙离子结合 收缩过程。目前的证据表明，SR受到 麻醉药，但可能不是收缩能力改变的主要部位。 概述的研究将检查氟烷、安氟醚、 和硫喷妥钠的亚细胞结构来观察变化 CA响应。结果将量化每种方法的相对影响。 麻醉剂对每个结构和可能的作用机制将是 假设的。所获得的数据将有助于制定药理学 预防麻醉剂引起的心脏抑制的干预措施 手术室。

Hypotension and cardiac arrest have been observed in patients anesthetized with the anesthetics, halothane, enflurane, and thiopental. These anesthetics are in wide clinical use today. Preliminary evidence suggests that these anesthetics might decrease the availability of Ca++ required for cardiac contraction. This project is designed to study the effect of the above mentioned anesthetics on structures in the myocardial cell that regulate Ca++ flux and control the intensity of contraction. The elements that will be studies are: the sarcolemma (SL), the outer membrane of the muscle cell; the sarcoplasmic reticulum (SR), a membranous network that controls the amount of mypolasmic Ca++; and troponin (Tn), a protein that responds to Ca++ binding by changing its shape and initiating the contractile process. Present evidence suggests that the SR is affected by anesthetics but may not be the major site of alteration of contractility. The studies outlined will examine the interaction of halothane, enflurane, and thiopental with the subcellular structures to observe alterations in Ca++ response. The results wll quantify the relative effect of each anesthetic on each structure and a possible mechanism of action will be postulated. The data obtained will help in formulating pharmacologic interventions to prevent anesthetic-induced cardiac depression in the operating room.