A STUDY OF DNA INTERCALATING AROMATIC AMINO AZIDES

我们将研究多环芳香氨基叠氮化合物的光化学 通过嵌入的方式与DNA结合。我们将使用低温矩阵红外， 用紫外-可见光谱、荧光光谱和电子顺磁共振光谱对其进行鉴定 叠氮乙锭和前列腺素光解制得中间体(S) 叠氮。我们将把这些叠氮化物光解成迷你双链 自我互补的二核苷酸。这些光反应的产物 将通过X射线结晶学进行鉴定。我们还将做好准备 叠氮乙啶和叠氮丙黄素的多氟衍生物。这 应大大简化叠氮光化学并增加绝对值 光生中间体的反应性。后一点将是 增加在结合部位共价连接的机会。我们会 研究这些多氟叠氮化物在溶液中和结合到迷你双链上 通过激光闪光光解获得标签的绝对寿命 中级的。

We will study the photochemistry of polycyclic aromatic amino azides which bind to DNA by intercalation. We will use low temperature Matrix IR, UV-Vis, fluorescence and EPR spectroscopy to identify the specific intermediate(s) produced by photolysis of ethidium azide and proflavine azide. We will photolyze these azides intercalated into mini duplexes of self complementary dinucleotides. The products of these photoreactions will be identified by X-ray crystallography. We will also prepare polyfluorinated derivatives of ethidium azide and proflavine azide. This should greatly simplify the azide photochemistry and increase the absolute reactivity of the photogenerated intermediates. The latter point will increase the chances of covalent attachment in the binding site. We will study these polyfluorinated azides in solution and bound to mini duplexes by laser flash photolysis to obtain the absolute lifetime of the labeling intermediate.