A STUDY OF DNA INTERCALATING AROMATIC AMINO AZIDES

DNA插入芳香氨基叠氮化物的研究

• 批准号: 3290558
• 负责人: MATTHEW PLATZ
• 金额: $ 8.73万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3290558
• 关键词: DNA; adduct; affinity labeling; amines; azides; chemical binding; covalent bond; cyclic compound; electron spin resonance spectroscopy; ethidium; flavins; fluorescence spectrometry; fluoridation; infrared spectrometry; laser spectrometry; nuclear magnetic resonance spectroscopy; nucleic acid structure; photochemistry; photolysis; ultraviolet spectrometry

We will study the photochemistry of polycyclic aromatic amino azides which bind to DNA by intercalation. We will use low temperature Matrix IR, UV-Vis, fluorescence and EPR spectroscopy to identify the specific intermediate(s) produced by photolysis of ethidium azide and proflavine azide. We will photolyze these azides intercalated into mini duplexes of self complementary dinucleotides. The products of these photoreactions will be identified by X-ray crystallography. We will also prepare polyfluorinated derivatives of ethidium azide and proflavine azide. This should greatly simplify the azide photochemistry and increase the absolute reactivity of the photogenerated intermediates. The latter point will increase the chances of covalent attachment in the binding site. We will study these polyfluorinated azides in solution and bound to mini duplexes by laser flash photolysis to obtain the absolute lifetime of the labeling intermediate.

我们将研究多环芳香族氨基叠氮化合物的光化学， 通过嵌入与DNA结合。 我们将使用低温矩阵红外， 紫外-可见，荧光和EPR光谱，以确定具体的 叠氮乙锭和原黄素光解产生的中间体 叠氮化物。 我们将光解这些叠氮化物插入到微型双链体， 自身互补的二核苷酸。 这些光反应的产物 将通过X射线晶体学进行鉴定。 还可获得 叠氮乙锭和叠氮原黄素的多氟化衍生物。这 应大大简化叠氮化物光化学，并增加绝对 光生中间体的反应性。 后一点将 增加结合位点中共价连接的机会。 我们将 在溶液中研究这些多氟叠氮化物并与微型双链体结合 通过激光闪光光解获得标记的绝对寿命 中间体