STRUCTURE AND ACTIVITY OF RIBOSOMAL GENES IN PHYSARUM

粘菌核糖体基因的结构和活性

• 批准号: 3288658
• 负责人: EDWARD M. JOHNSON
• 金额: $ 9.81万
• 依托单位: MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3288658
• 关键词: DNA directed RNA polymerase; analytical ultracentrifugation; chemical fingerprinting; eukaryote; gene expression; genetic manipulation; genetic mapping; genetic transcription; microinjections; nucleic acid reconstitution; nucleic acid sequence; radiotracer; ribosomes; tissue /cell culture

This project concerns the genes coding for ribosomal RNA in the lower eukaryote Physarum polycephalum. These genes are located on a giant palindromic DNA molecule which is predominantly extrachromosomal in plasmodia. They can be isolated in milligram quantities together with their associated histones and regulatory proteins. We have cloned restriction fragments of the ribosomal genes and intend to sequence important transcriptional regulatory regions and intervening sequences. We shall elucidate the origin and functions of specific single-strand gaps and tightly-bound proteins at the rDNA termini. We shall examine the association of the rDNA palindrome with chromosomal DNA components. Through use of fluorescent-labeled histone we shall determine the nature of nucleosome structural changes which occur during transcription. We shall continue to examine the location and structure of nucleosomes on transcriptional regulatory sequences and on transcribed and non-transcribed rDNA regions.

该项目涉及低等真核生物中编码核糖体 RNA 的基因 多头绒泡菌。 这些基因位于巨大的回文 DNA 上 疟原虫中主要存在于染色体外的分子。 他们可以是 以毫克量与其相关的组蛋白一起分离， 调节蛋白。 我们克隆了核糖体的限制性片段 基因并打算对重要的转录调控区域进行测序 干预序列。 我们将阐明特定的起源和功能 rDNA 末端的单链间隙和紧密结合的蛋白质。 我们将 检查 rDNA 回文与染色体 DNA 成分的关联。 通过使用荧光标记的组蛋白，我们将确定 转录过程中发生的核小体结构变化。 我们将 继续研究核小体在转录中的位置和结构 调控序列以及转录和非转录 rDNA 区域。