NMR STUDIES OF BIOLOGICAL MEMBRANES
生物膜的核磁共振研究
基本信息
- 批准号:3291312
- 负责人:
- 金额:$ 15.91万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1985
- 资助国家:美国
- 起止时间:1985-09-30 至 1991-03-31
- 项目状态:已结题
- 来源:
- 关键词:Halobacteriaceae Raman spectrometry antibiotics bacteriorhodopsins cations ion transport ionophores membrane model membrane proteins membrane structure metal complex molecular dynamics nuclear magnetic resonance spectroscopy passive transport photochemistry protein sequence protein structure function stoichiometry transport proteins
项目摘要
Solid-state nuclear magnetic resonance spectroscopy and resonance Raman
spectroscopy are to be used as non-perturbative means of studying the
structures, dynamics and functional mechanisms of proteins and peptides
involved in membrane transport. In particular, we plan to study the
purple membrane fragments of the plasma membrane of the halobacterium
halobium, which contains a single protein that executes light driven proton
transport, and model membranes containing enniatin ionophores, which
facilitate passive cation transport. Samples will be isotopically enriched
at specific sites to enhance NMR signals of interest, suppress interfering
NMR signals, and to induce informative shifts in the resonance Raman
spectrum. In the case of the purple membrane, we hope to learn which
groups are exposed to the aqueous environment in native and bleached
samples, what the pK's of the ionizable residues are, which ionizable
groups change their state of protonation during the photo reaction cycle,
which groups are in direct interaction with the chromophore, and what types
of moleculr motion occur in various regions of the protein. In the case of
the enniatin ionophores, we hope to learn what the stoichiometries and
structures of the metal ion complexes are and what types of molecular
motion occur in the aliphatic and aromatic side chains. In addition to
illuminating the properties of these particular systems, it is hoped that
these biophysical studies will provide models for other membrane transport
systems and integral membrane proteins in general. Information about such
proteins is ultimately important to our understanding of normal and
pathological cell regulation and function.
固态核磁共振波谱和共振拉曼
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JUDITH HERZFELD其他文献
JUDITH HERZFELD的其他文献
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{{ truncateString('JUDITH HERZFELD', 18)}}的其他基金
Structure and Interactions of Gas Vesicles by SSNMR
通过 SSNMR 观察气体囊泡的结构和相互作用
- 批准号:
7179309 - 财政年份:2003
- 资助金额:
$ 15.91万 - 项目类别:
Structure and Interactions of Gas Vesicles by SSNMR
通过 SSNMR 观察气体囊泡的结构和相互作用
- 批准号:
6742443 - 财政年份:2003
- 资助金额:
$ 15.91万 - 项目类别:
Structure and Interactions of Gas Vesicles by SSNMR
通过 SSNMR 观察气体囊泡的结构和相互作用
- 批准号:
6601244 - 财政年份:2003
- 资助金额:
$ 15.91万 - 项目类别:
Structure and Interactions of Gas Vesicles by SSNMR
通过 SSNMR 观察气体囊泡的结构和相互作用
- 批准号:
6856474 - 财政年份:2003
- 资助金额:
$ 15.91万 - 项目类别:
Structure and Interactions of Gas Vesicles by SSNMR
通过 SSNMR 观察气体囊泡的结构和相互作用
- 批准号:
7022932 - 财政年份:2003
- 资助金额:
$ 15.91万 - 项目类别:
SELF-ASSEMBLY AND SPONTANEOUS ORDER IN CROWDED SOLUTIONS
拥挤解决方案中的自组装和自发秩序
- 批准号:
2218195 - 财政年份:1985
- 资助金额:
$ 15.91万 - 项目类别:
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