STRATEGY FOR THE CHARACTERIZATION OF THE HUMAN GENOME

人类基因组表征策略

• 批准号: 3287198
• 负责人: LEONARD S LERMAN
• 金额: $ 12.7万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3287198
• 关键词: DNA; biological polymorphism; complementary DNA; gel electrophoresis; genetic manipulation; genetic mapping; genome; human tissue; image processing; nucleic acid sequence; nucleic acid structure; nucleobase; temperature

The program is concerned with the detection and analysis of sequence variations in the human genome, particularly those responsible for genetic disease or useful in following those variants. We will depend largely on the system of sequence-defined DNA separations of Fischer and Lerman and extend the system, both in its experimental capability and in theoretical basis of its function. We will also pursue certain structural and thermodynamic questions, particularly those bearing on the relations between sequence and the stability of the helix with respect to melting.

该程序涉及序列的检测和分析 人类基因组中的变异，特别是那些负责遗传 疾病或用于遵循这些变体。 我们将主要依靠 Fischer和Lerman的序列定义DNA分离系统， 扩展系统，无论是在其实验能力和理论 其功能的基础。 我们还将寻求一些结构性和 热力学问题，特别是那些关系 序列和螺旋相对于解链的稳定性之间。

项目成果

Searching for gene defects by denaturing gradient gel electrophoresis.

通过变性梯度凝胶电泳寻找基因缺陷。

• DOI: 10.1101/sqb.1986.051.01.034
• 发表时间: 1986
• 期刊: Cold Spring Harbor symposia on quantitative biology
• 作者: Lerman,LS;Silverstein,K;Grinfeld,E
• 通讯作者: Grinfeld,E

Relaxation effects in the gel electrophoresis of DNA in intermittent fields.

间歇场中 DNA 凝胶电泳的弛豫效应。

• DOI: 10.1002/bip.360280807
• 发表时间: 1989
• 期刊: Biopolymers
• 影响因子: 2.9
• 作者: Jamil,T;Frisch,HL;Lerman,LS
• 通讯作者: Lerman,LS

Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis.

• DOI: 10.1016/0076-6879(87)55032-7
• 发表时间: 1987
• 期刊: Methods in enzymology
• 作者: L. Lerman;K. Silverstein