PARACOCCUS DENITRIFICANS IRON TRANSPORT APPARATUS

副球菌脱氮铁转运装置

• 批准号: 3286716
• 负责人: Raymond Joseph Bergeron
• 金额: $ 11.64万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3286716
• 关键词: acylation; alkylating agents; azides; bacteria; chelating agents; gallium; iron metabolism; ligands; membrane permeability; nitrene; photolysis; tritium

This program will focus on the isolation of a component of the Paracoccus denitrificans iron transport apparatus. The major aim of the program is to selectively and irreversibly label the microorganism's siderophore surface receptor in order to facilitate its isolation and eventual structural elucidation. The hope is that the isolation of this receptor will provide us with a better understanding of how the organism utilizes its siderophore, parabactin, in the assimilation of iron. The program will be divided into two segments: 1) the design and synthesis of a parabactin receptor label and 2) the fixing of the parabactin label to the Paracoccus denitrificans parabactin receptor. The preliminary evidence presented in the text to follow strongly suggests that the program has a high index of success. We have already demonstrated our expertise in synthesis of related systems as well as our ability to handle the associated microbiological manipulations.

本程序将重点介绍Paracoccus组件的隔离 脱氧核糖核酸铁质输送装置。该计划的主要目的是 选择性和不可逆地标记微生物的铁载体表面 受体，以便于其分离和最终的结构 澄清。希望这种受体的分离将提供 让我们更好地了解这种有机体是如何利用其 铁载体，副肌动蛋白，在铁的同化中。该计划将是 分为两个部分：1)副肌动蛋白的设计和合成 受体标记和2)副肌动蛋白标记在副球囊上的固定 反硝化细菌副肌动蛋白受体。中提出的初步证据 后面的文字强烈表明，该计划有很高的指数 成功。我们已经展示了我们在合成 相关系统以及我们处理相关问题的能力 微生物操作。