PITUITARY CELL CULTURE: ESTROGEN CONTROL OF FSH

绵羊垂体细胞培养中促性腺激素受雌激素的影响 容易测量的方法。雌激素可(1)降低自发性FSH 分泌(在2至30小时内超过90%)和(2)增加促性腺激素 在6小时内对促性腺激素释放激素敏感(约10的负10次方M的20倍 LHRH)。我们的研究旨在将绵羊促性腺激素细胞用于细胞培养 雌激素在促性腺激素细胞中的作用及促性腺激素的调控 一般的分泌，重点是促卵泡刺激素。 近期目标是描述FSH生物合成的以下几个方面(1) 信使核糖核酸的产生(使用来自克隆的cDNA或编码基因的杂交探针 对于FSH亚基)和(2)亚基mRNAs的翻译 加工和分泌。雌激素对每一步的影响将是 下定决心。还将努力评估其他生理学方面的 重要物质，如抑制素和LHRH，或FSH生物合成和 分泌物。最后，将努力评估LHRH的约束力或 从促性腺激素角度看，内化可能是雌激素作用的部位 对LHRH敏感。这些研究还可能带来关于以下方面的基本知识 LHRH结合，进而可以用来开发有效的方法 使用LHRH特异性方法富集促性腺激素。 详细了解雌激素在绵羊促性腺激素细胞中的作用可能会增加 对真核基因调控的一般了解，并可能在临床上 水平，为治疗这些乳腺癌提供有用的信息 对雌激素敏感(60%至70%)。此外，清楚地认识到 雌激素、抑制素等物质对促性腺激素分泌的作用 可能会产生与(1)纠正人类生殖功能障碍有关的数据，(2) 可靠、安全的节育方法；(3)提高生育率 饲养供食用的动物的能力。

Ovine gonadotrophs in pituitary cell culture are affected by estrogens in two ways which are easily measured. Estrogens can (1) decrease spontaneous FSH secretion (greater than 90% between 2 and 30 h) and (2) increase gonadotroph sensitivity to GNRH within 6 h (approx. 20-fold at 10 to the minus 10th power M LHRH). Our research is designed to use ovine gonadotrophs in cell culture for studying estrogen action in gonadotrophs and the control of gonadotrophin secretion in general, with an emphasis on FSH. Immediate aims are to characterize the following aspects of FSH biosynthesis (1) mRNA production (using hybridization probes from cloned cDNAs or genes coding for FSH subunits) and (2) translation of the subunit mRNAs with subsequent processing and secretion. The effects of estrogen on each step will be determined. Efforts will also be made to evaluate other physiologically important substances, such as inhibin and LHRH, or FSH biosynthesis and secretion. Finally, efforts will be made to evaluate LHRH binding or internalization as a possible site of estrogen action in terms of gonadotroph sensitization to LHRH. These studies may also lead to basic knowledge about LHRH binding which may, in turn, be used to develop effective methods for enriching gonadotrophs by using LHRH-specific methods. Detailed knowledge of estrogen action in ovine gonadotrophs may add to the general understanding of eucaryotic gene regulation and may, at a clinical level, provide information useful for treating those mammary cancers which are estrogen sensitive (60 to 70%). Furthermore, a clear understanding of the actions of estrogen, inhibin, and other substances on gonadotrophin secretions may yield data relevant to (1) correction of human reproductive disfunction, (2) reliable and safer birth control methods, and (3) increasing reproductive capacity of animals raised for food.