PITUITARY CELL CULTURE: ESTROGEN CONTROL OF FSH

垂体细胞培养中的绵羊促性腺激素细胞受雌激素的影响， 容易衡量的方法。 雌激素可（1）降低自发性FSH 分泌（在2和30小时之间大于90%）和（2）增加促性腺激素 6小时内对GNRH的敏感性（约20-以10的负10次方M折叠 LHRH）。 我们的研究旨在使用绵羊促性腺激素细胞培养， 研究雌激素在促性腺激素细胞中的作用和促性腺激素的控制 一般分泌，重点是FSH。 当前的目标是描述FSH生物合成的以下方面（1） mRNA产生（使用来自克隆的cDNA或编码 FSH亚基）和（2）亚基mRNA的翻译， 加工和分泌。 雌激素对每一步的影响将是 测定 还将努力评估其他生理学 重要的物质，如卵泡刺激素和LHRH，或FSH生物合成， 分泌物 最后，将努力评估LHRH的约束力， 内化作为雌激素促性腺激素作用可能位点 对LHRH敏感。 这些研究也可能导致基本知识， LHRH结合，这反过来又可以用来开发有效的方法， 通过使用LHRH特异性方法富集促性腺激素细胞。 详细了解雌激素在绵羊促性腺激素细胞中的作用， 真核基因调控的一般理解，并可能在临床上 水平，为治疗那些乳腺癌提供有用的信息， 雌激素敏感（60 - 70%）。 此外，要清楚地了解 雌激素、雌二醇和其他物质对促性腺激素分泌的作用 可以产生与以下相关的数据：（1）人类生殖功能障碍的矫正，（2） 可靠和安全的避孕方法，（3）增加生殖 饲养动物的能力。

Ovine gonadotrophs in pituitary cell culture are affected by estrogens in two ways which are easily measured. Estrogens can (1) decrease spontaneous FSH secretion (greater than 90% between 2 and 30 h) and (2) increase gonadotroph sensitivity to GNRH within 6 h (approx. 20-fold at 10 to the minus 10th power M LHRH). Our research is designed to use ovine gonadotrophs in cell culture for studying estrogen action in gonadotrophs and the control of gonadotrophin secretion in general, with an emphasis on FSH. Immediate aims are to characterize the following aspects of FSH biosynthesis (1) mRNA production (using hybridization probes from cloned cDNAs or genes coding for FSH subunits) and (2) translation of the subunit mRNAs with subsequent processing and secretion. The effects of estrogen on each step will be determined. Efforts will also be made to evaluate other physiologically important substances, such as inhibin and LHRH, or FSH biosynthesis and secretion. Finally, efforts will be made to evaluate LHRH binding or internalization as a possible site of estrogen action in terms of gonadotroph sensitization to LHRH. These studies may also lead to basic knowledge about LHRH binding which may, in turn, be used to develop effective methods for enriching gonadotrophs by using LHRH-specific methods. Detailed knowledge of estrogen action in ovine gonadotrophs may add to the general understanding of eucaryotic gene regulation and may, at a clinical level, provide information useful for treating those mammary cancers which are estrogen sensitive (60 to 70%). Furthermore, a clear understanding of the actions of estrogen, inhibin, and other substances on gonadotrophin secretions may yield data relevant to (1) correction of human reproductive disfunction, (2) reliable and safer birth control methods, and (3) increasing reproductive capacity of animals raised for food.