A New Platform for Real Time Analysis of Metabolic Processes in Live Cells

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We request an Agilent Seahorse XF Pro analyser interfaced with Cytation 1 imager and software for high throughput real-time analysis of metabolic processes in live cells.Metabolic phenotyping is the term given to the comprehensive analysis of biological samples, such as body fluids (eg urine, blood, saliva), tissues (eg muscle, epithelial, nervous), or cells (eg bacteria, yeast, mammalian) to understand physiological (healthy) or pathological (disease) states. Analytical technologies for metabolic phenotyping of living cells in real-time are essential to fully understand biological processes in their near-to-native state. The routine analysis of cellular mechanisms has the potential to improve our understanding of cell growth and implications of cell aging, impact of environmental changes on cellular health, initiation of disease in healthy cells, effect of drugs or treatments on diseased cells, and impact of interventions on cellular homeostasis. Majority of our existing understanding of metabolic phenotypes involve cell disruption (breaking cells open) to access and analyse its metabolome, or the analysis of the cellular environment to deduce internal cellular bioprocesses. Neither of these approaches accurately represent the native state of metabolism in living cells. It is therefore essential to probe cellular metabolic processes in live cells to better understand biology, diseases, aging, as well as to improve the use of cells in engineering organisms for valuable commodities (such as anti-cancer drugs, vaccines, flavours and fragrances, plastic alternatives, etc).The Agilent Seahorse XF Pro analyser is an instrument that can measure vital cellular processes in live cells, without using disruptive (cell lysis) techniques. The instrument preserves cell integrity during analysis and allows repeat measurements to be taken on the same cells over time, providing an unprecedented depth in metabolic phenotyping of live cells. Additionally, the instrument is easy-to-use, has high throughput, is compatible with a wide variety of cell types, requires minimal cleaning and calibration, and is user-friendly with an intuitive software platform, making it an ideal instrument for multi-user environments, such as the EdinOmics facility. By adding this capability to EdinOmics existing portfolio of analytical technologies, we will be able to provide world leading researchers at the University of Edinburgh with a high throughput metabolic phenotyping technology to improve research productivity, minimise experiment costs, reduce time to results and speed up technology translation to market.