BIOANALYTICAL MICROSEPARATIONS, DETECTION AND PERFUSION

这项提议涉及到发展和表征 以微柱分离为主的生物分析方法 技巧。我们感兴趣的系统本质上是神经化学的。 这是一个面向方法开发的建议。我们计划调查 碳膜电极作为柱上电化学检测器的应用 适用于微柱液体分离方案。这个探测器是非常 敏感度，应适用于任何大小的列和应用程序 毛细管区带电泳法是可行的。毛细管区的使用 涉及神经化学有趣化合物的方案的电泳法 计划同时使用激光荧光和电化学检测。 最后，我们建议使用电渗流来获得极小的 体积样品并将这些样品“注入”到毛细管中 电泳柱。一个类似的方案将被用于在体内进行 大鼠灌流实验。这些实验可以通过以下方式进行 并排的毛细血管直径只有几微米。

This proposal concerns the development and characterization of bioanalytical methods based primarily upon microcolumn separation techniques. The systems we are interested in are neurochemical in nature. This is a methods development oriented proposal. We plan to investigate the use of a carbon film electrode as an on-column electrochemical detector for microcolumn liquid separation schemes. This detector is extremely sensitive, should be applicable to any size column and application to capillary zone electrophoresis is feasible. The use of capillary zone electrophoresis for schemes involving neurochemically interesting compounds is planned with both laser fluorescence and electrochemical detection. Lastly, we propose to use electroosmotic flow to acquire extremely small volume samples and to "inject" these samples onto the capillary electrophoresis column. A similar scheme will be used to carry out in vivo perfusion experiments on rats. These experiments can be carried out with side-by-side capillaries drawn out to only a few microns in diamter.