AUTOMATED HIGH SPEED DNA SEPARATIONS

自动化高速 DNA 分离

基本信息

  • 批准号:
    6181646
  • 负责人:
  • 金额:
    $ 17.24万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1998
  • 资助国家:
    美国
  • 起止时间:
    1998-07-01 至 2001-12-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION: Development of high throughput DNA sampling, separation, and detection strategies are described for use in sequencing and genotyping. New technology is proposed for massively parallel, low-volume DNA separations. This will be accomplished by combining capillary sample introduction parallel separations in microfabricated chips, and electrochemical array detection to provide a single integrated system for separation and detection. Ultimately, this will be connected via a sampling capillary to an array of nanovials for introduction of large numbers of samples. The proposal is divided into four major parts which include 1) optimization of separations of DNA fragments in ultrathin channels with sieving buffers, 2) development of an improved interface to allow efficient sample transfer across the capillary-channel interface, 3) development of electrochemical array detection using Ru(bpy)32+ as an electrophore for dsDNA and ssDNA, and 4) application of the capillary transfer method and electrochemical array detection to DNA separations on chips. This will provide the means to rapidly place multiple samples in a very large number of channels (1000 parallel channels is feasible) on a single chip. The proposed technology promises to increase sample throughput by 10- to 50-fold and reduce sample size by 2 to 3 orders of magnitude relative to currently available systems.
描述:开发高通量DNA取样,分离和

项目成果

期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
专利数量(0)

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ANDREW G EWING其他文献

ANDREW G EWING的其他文献

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{{ truncateString('ANDREW G EWING', 18)}}的其他基金

Microfluidic Systems to Address Networks of Neurons
用于处理神经元网络的微流体系统
  • 批准号:
    7432585
  • 财政年份:
    2006
  • 资助金额:
    $ 17.24万
  • 项目类别:
Microfluidic Systems to Address Networks of Neurons
用于处理神经元网络的微流体系统
  • 批准号:
    7033561
  • 财政年份:
    2006
  • 资助金额:
    $ 17.24万
  • 项目类别:
Microanalytical Methods for Drosophila Neurochemistry
果蝇神经化学的微量分析方法
  • 批准号:
    7666748
  • 财政年份:
    2006
  • 资助金额:
    $ 17.24万
  • 项目类别:
Microanalytical Methods for Drosophila Neurochemistry
果蝇神经化学的微量分析方法
  • 批准号:
    7477698
  • 财政年份:
    2006
  • 资助金额:
    $ 17.24万
  • 项目类别:
Microanalytical Methods for Drosophila Neurochemistry
果蝇神经化学的微量分析方法
  • 批准号:
    7135939
  • 财政年份:
    2006
  • 资助金额:
    $ 17.24万
  • 项目类别:
Microfluidic Systems to Address Networks of Neurons
用于处理神经元网络的微流体系统
  • 批准号:
    7238515
  • 财政年份:
    2006
  • 资助金额:
    $ 17.24万
  • 项目类别:
Microanalytical Methods for Drosophila Neurochemistry
果蝇神经化学的微量分析方法
  • 批准号:
    7269508
  • 财政年份:
    2006
  • 资助金额:
    $ 17.24万
  • 项目类别:
Microfluidic Systems to Address Networks of Neurons
用于处理神经元网络的微流体系统
  • 批准号:
    7628456
  • 财政年份:
    2006
  • 资助金额:
    $ 17.24万
  • 项目类别:
Electroanalytical Probes of Exocytosis
胞吐作用的电分析探针
  • 批准号:
    6331685
  • 财政年份:
    1998
  • 资助金额:
    $ 17.24万
  • 项目类别:
Analytical Techniques for Exocytosis
胞吐作用的分析技术
  • 批准号:
    7098910
  • 财政年份:
    1998
  • 资助金额:
    $ 17.24万
  • 项目类别:
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