ENZYMIC SYNTHESIS OF BIOACTIVE AGENTS IN ORGANIC MEDIA

有机介质中生物活性剂的酶法合成

• 批准号: 3296994
• 负责人: ALEXANDER M KLIBANOV
• 金额: $ 15.36万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-20 至 1991-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3296994
• 关键词: B lymphocyte; aminoacid; chemical synthesis; chromosome translocation; complementary DNA; enzyme induction /repression; genetic recombination; genome; immunoglobulin G; immunoglobulin genes; immunoglobulin structure; molecular biology; neoplasm /cancer genetics; nucleic acid sequence; organic chemicals; peptides; peripheral blood vessel; protein signal sequence; transfection

The goal of this study is to identify the functional diversity (D) segments of the human immunoglobulin heavy chain (IgH) gene and to obtain insights into the molecular mechanism of VDJ recombination in this gene. Experiments are proposed herein to study the entire repertoire of human functional D segments by molecular cloning of functionally rearranged IgH sequences and to characterize the genomic organization of the whole repertoire of D segments. An in vivo assay system of DJ and VDJ recombination will be developed and used to elucidate the molecular basis of VDJ recombination. For this purpose, a series of DNA constructs will be made including those with mutated 7mer-9mer signal sequences and spacer sequences and recombination activity will be assayed in the in vivo system. Analyses are also proposed to determine the underlying basis of V segment joining to the DJ complex, by introducing a DNA construct into the assay system.

这项研究的目标是确定功能多样性(D) 人免疫球蛋白重链(IgH)基因片段 并深入了解VDJ的分子机制 这种基因的重组。在此提出的实验是为了 通过以下方式研究人类功能D片段的全部内容 功能性重排免疫球蛋白IgH序列的分子克隆及鉴定 描述整个曲目的基因组结构 D段。DJ和VDJ体内检测系统的研制 重组将被开发并用于阐明 VDJ重组的分子基础。为此，我们推出了一系列 的DNA结构，包括突变的DNA结构 7mer-9mer信号序列和间隔区序列以及 重组活性将在体内系统中进行检测。 还建议进行分析，以确定V的潜在基础 通过引入DNA连接到DJ复合体的片段 构建到检测系统中。