ENZYMIC SYNTHESIS OF BIOACTIVE AGENTS IN ORGANIC MEDIA

有机介质中生物活性剂的酶法合成

• 批准号: 3296995
• 负责人: ALEXANDER M KLIBANOV
• 金额: $ 15.68万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-20 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3296995
• 关键词: B lymphocyte; aminoacid; chemical synthesis; chromosome translocation; complementary DNA; enzyme induction /repression; genetic recombination; genome; immunoglobulin G; immunoglobulin genes; immunoglobulin structure; molecular biology; neoplasm /cancer genetics; nucleic acid sequence; organic chemicals; peptides; peripheral blood vessel; protein signal sequence; transfection

The goal of this study is to identify the functional diversity (D) segments of the human immunoglobulin heavy chain (IgH) gene and to obtain insights into the molecular mechanism of VDJ recombination in this gene. Experiments are proposed herein to study the entire repertoire of human functional D segments by molecular cloning of functionally rearranged IgH sequences and to characterize the genomic organization of the whole repertoire of D segments. An in vivo assay system of DJ and VDJ recombination will be developed and used to elucidate the molecular basis of VDJ recombination. For this purpose, a series of DNA constructs will be made including those with mutated 7mer-9mer signal sequences and spacer sequences and recombination activity will be assayed in the in vivo system. Analyses are also proposed to determine the underlying basis of V segment joining to the DJ complex, by introducing a DNA construct into the assay system.

本研究的目的是确定功能多样性（D） 人免疫球蛋白重链（IgH）基因的片段 并深入了解VDJ的分子机制 在这个基因中重组。 本文提出实验以 研究人类功能性D片段的全部库， 功能重排的IgH序列的分子克隆， 描述了整个基因组的基因组结构， D段。 DJ和VDJ的体内测定系统 重组将被开发和用于阐明 VDJ重组的分子基础。 为此，一系列 的DNA构建体，包括那些突变的 7 mer-9 mer信号序列和间隔区序列，以及 将在体内系统中测定重组活性。 还提出了分析，以确定潜在的基础V 通过引入DNA， 构建到测定系统中。