FUNCTION OF THE DECIDUAL TISSUE

这项建议的总体目标是阐明蜕膜的作用。 组织在孕期黄体功能调节中的作用 假孕大鼠。第一种方法，使用移植和 放射免疫测定法，将测定孕妇蜕膜组织(DT) 与假孕动物的DT一样，大鼠在体内维持孕酮合成 缺少催乳素。利用切除了脑垂体的动物，我们将确定 蜕膜组织的促黄体作用是否与 黄体生成素或雌激素。蜕膜组织刺激的可能性 催乳素，黄体生成素和雌二醇受体的生成 使用放射感受器技术进行研究。最后，我们将确定 DT激素是否像大鼠胎盘催乳素那样与同一受体结合 如果是这样的话，DT-激素的水平将在 DT和血清中。

The over-all objective of this proposal is to elucidate the role of the decidual tissue in the regulation of corpus luteum function in pregnant and pseudopregnant rats. The first approach, using transplantation and radioimmunoassay, will be determine whether the decidual tissue (DT) of pregnant rats, like the DT of pseudopregnant animals, sustain progesterone synthesis in the absence of prolactin. Using hypophysectomized animals we will determine whether the luteotropic effect of the decidual tissue is related to the presence of LH or estrogen. The possibility that the decidual tissue stimulates, like prolactin, the generation of both LH and estradiol receptor will then be investigated using a radioreceptor technique. Finally, we will determine whether the DT-hormone bind, as does rat placental lactogen to the same receptor site than prolactin, and if so the levels of the DT-hormone will be measured in the DT and in the serum.