SECRETION AND ACTION OF DECIDUAL LUTEOTROPIN

本建议的总体目标是进一步了解 分泌和作用的蜕膜促黄体激素，催乳素， 像激素分泌的蜕膜组织怀孕和 假孕大鼠 第一个目标是建立一个直接的 蜕膜促黄体激素对黄体和卵泡的作用 类固醇生成 在这项研究中，卵泡和黄体细胞 将被从蜕膜细胞流出的培养基灌注 灌注，或将与高度纯化的激素一起孵育。 使用体外和体内方法，我们将研究 蜕膜促黄体激素局部作用于蜕膜和 邻近的滋养层细胞控制糖原的产生， 蜕膜和滋养层细胞的类固醇生成。 第三 具体目的是确定蜕膜的个体发育 在整个蜕膜生命周期中促黄体生成素的产生 组织. 到目前为止，除了女人和老鼠， 已知PRL样物质的产生是否是 蜕膜组织的激素功能。 最后一个目的是 研究将确定是否在其他物种中， 蜕膜化伴随着床，催乳素样 激素由蜕膜组织产生。 这将 在具有以下特征的物种中建立蜕膜作为内分泌腺 血绒膜胎座式 我们也会调查 在某些物种中， 促黄体活性

The overall objective of this proposal is to gain further insight into the secretion and action of decidual luteotropin, a prolactin- like hormone secreted by the decidual tissue of pregnant and pseudopregnant rats. The first aim will be to establish a direct action of decidual luteotropin on luteal and follicular steroidogenesis. For this investigation, follicles and luteal cells will be either perifused with medium flowing from a decidual cell perifusion, or will be incubated with highly purified hormone. Using in vitro and in vivo approaches, we will investigate the possibility that decidual luteotropin acts locally on decidual and neighboring trophoblastic cells to control glycogen production by the decidua, and steroidogenesis by trophoblastic cells. The third specific aim is designed to determine the ontogeny of decidual luteotropin production throughout the life span of the decidual tissue. To date, with the exception of women and rats, it is not known whether the production of a PRL-like substance is a hormonal function of the decidual tissue. The last aim of this study will be to determine whether in other species in which decidualization accompanies implantation, prolactin-like hormones are produced by the decidual tissue. This would establish the decidua as an endocrine gland in species with hemochorial placentation. We will also investigate the possibility that in some of these species, decidual prolactin possesses luteotropic activity.