HUMAN CHORIONIC AND OTHER GONADOTROPINS

人绒毛膜促性腺激素和其他促性腺激素

• 批准号: 3310976
• 负责人: OM P BAHL
• 金额: $ 19.06万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310976
• 关键词: affinity chromatography; carbohydrate sequence; chemical group; chemical structure function; chorionic gonadotropin; cyclic AMP; female antifertility drug; follicle stimulating hormone; glycosyltransferase; human subject; immunofluorescence technique; luteinizing hormone; placenta; radioimmunoassay; spermicide; steroid hormone biosynthesis

The work is related to studies on the structure, function and biosynthesis of gonadotropins. The structural studies include the assignment of disulfide bonds in the alpha and beta subunits of human chorionic gonadotropins (hCG), structures of the carbohydrate units of ovine and bovine luteinizing hormones (o, bLH) and pregnant mare serum gonadotropin (PMSG). All the disulfide bonds in hCG-alpha and hCG-beta have been assigned. The carbohydrate structures of oLH and bLH have been determined. It has been found that the carbohydrate structural pattern in them is quite different from that present in other glycoproteins. The work on the carbohydrate structure of PMSG will be initiated. The structure and function studies have focused primarily on the modification of hCG-beta to obtain a highly specific antigen suitable for producing an hCG specific antibody. Also, these studies have included the determination of the role of carbohydrate in the function of hCG. The removal of carbohydrate from hCG has resulted in the derivatives which are inhibitors of adenyl cyclase and steriodogenesis. The mechanism of action of the derivatives will be tested. The biosynthetic studies have involed the determination of the structure of oligosaccharide-lipid intermediate -a precursor of carbohydrate unit in glycoproteins. Finally, the work on the isolation and characterization of hCG/hLH receptor from rat ovaries is in progress.

这项工作与对其结构、功能和生物合成的研究有关。 促性腺激素。结构研究包括二硫键的归属。 在人绒毛膜促性腺激素(HCG)的α和β亚基中， 绵羊和牛促黄体激素碳水化合物单位的结构 (O，BLH)和孕马血清促性腺激素(PMSG)。所有的二硫键 其中hCG-α和hCG-β已被指认。的碳水化合物结构 测定了OLH和BLH值。人们已经发现，碳水化合物 它们的结构模式与其他国家的结构模式大不相同 糖蛋白。关于PMSG的碳水化合物结构的工作将是 已启动。对其结构和功能的研究主要集中在 人绒毛膜促性腺激素-β基因修饰获得高特异性抗原 产生hCG特异性抗体。此外，这些研究还包括 测定碳水化合物在hCG功能中的作用。去掉 从人绒毛膜促性腺激素中提取的碳水化合物产生了一些衍生物，它们是 腺苷环化酶与甾体碘的发生。衍生物的作用机理 将会受到考验。生物合成研究涉及到对 碳水化合物前体低聚糖-脂类中间体的结构 单位为糖蛋白。最后，对分离和表征工作进行了研究 大鼠卵巢hCG/HLH受体的研究正在进行中。