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BIOSYNTHESIS OF BOVINE LUTEINIZING HORMONE

牛黄体生成素的生物合成

基本信息

批准号：
3311926
负责人：
OM P BAHL
金额：
$ 4.95万
依托单位：
STATE UNIVERSITY OF NEW YORK AT BUFFALO
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
起止时间：
1978-12-01 至 1986-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3311926
关键词：
N acetylgalactosamine asparagine carbohydrate biosynthesis carbohydrate structure chromatography cyclic AMP immunochemistry luteinizing hormone oligosaccharides peptide hormone biosynthesis pituitary gonadal axis radiotracer steroid hormone

项目摘要

Gonadotropins (LH, FSH and hCG) produced by pituitary and placenta, are key hormones involved in human reproduction. This proposal related to the structure, function and biosynthesis of bLH is a continuation of our ongoing research. The carbohydrate structure in bLH is unique in having a 54-0 substituted N-acetylgalactosamine at the nonreducing end of the asparagine-linked carbohydrate units. The identity of the substituent has not been established. The specific objects of this proposal are: (1) Identification of the substituent on N-acetylgalacosamine. (2) Characterization of the enzymes which incorporate the substituent and N-acetylgalactosamine. (3) Study of the mechanism of assembly of the oligosaccharide-lipid involved in the biosynthesis of bLH carbohydrate by the isolation of major intermediates. (4) Study of the processing of the oligosacchardie after its transfer to the protein by the isolation of various intermediates and the addition of peripheral sugars. (5) Attempt on the total in vitro synthesis of bLH using deglycosylated bLH-Alpha and bLH-Beta, transfer of the oligosaccharide from oligosacharide-lipid intermediate followed by the complete processing of the oligosaccharide. (6) Study of the effect of steroids, LH-releasing factor and cAMP on the synthesis and secretion of bLH. (7) Study of the role of carbohydrate in the biological function of the hormone by the removal of the carbohydrate from the individual subunits by an enzyme oligosaccharide amidohydrolase or by the synthesis of the deglycosylated bLH in calf pituitary slices in the presence of tunicamycin. Information developed from this work will help in designing experiments for the study of human LH, which will add to our understanding of the regulation in human fertility.

垂体和胎盘产生的促性腺激素（LH，FSH和hCG）是关键与人类生殖有关的激素这项建议涉及到 bLH结构、功能和生物合成是我们研究的继续正在进行的研究。 bLH中的碳水化合物结构是独特的， 54-0取代的N-乙酰半乳糖胺的非还原端，天冬酰胺连接的碳水化合物单元。取代基的身份具有尚未建立。这项建议的具体目标是：（1） N-乙酰半乳糖胺上取代基的鉴定。（二）掺入取代基的酶的表征和 N-乙酰半乳糖胺 (3)聚乙烯聚合物的组装机理研究寡糖-脂质参与bLH碳水化合物的生物合成，主要中间体的分离。 (4)研究的过程中，寡糖在其转移到蛋白质后通过分离各种中间体和外围糖的添加。 (5)尝试使用去糖基化的bLH-α和 bLH-β，从寡糖-脂质转移寡糖中间体，然后是寡糖的完全加工。 (6)类固醇激素、LH释放因子和cAMP对大鼠脑缺血再灌注损伤作用的研究 bLH合成和分泌。 (7)碳水化合物在糖尿病中作用的研究激素的生物学功能通过去除碳水化合物通过寡糖酰胺水解酶从各个亚基中分离，或通过牛脑垂体片去糖基化bLH的合成，衣霉素的存在。从这项工作中获得的信息将有助于设计研究人类LH的实验，这将增加我们的了解人类生育能力的调节。