SITE-SPECIFIC ENERGETICS OF PROTEIN-DNA INTERACTIONS

蛋白质-DNA 相互作用的位点特异性能量

• 批准号: 3296228
• 负责人: Gary K Ackers
• 金额: $ 11.53万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3296228
• 关键词: DNA; DNA footprinting; acidity /alkalinity; autoradiography; bacteriophage lambda; bioenergetics; chemical binding; gel filtration chromatography; genetic regulation; image processing; ionic strengths; mathematical model; mutant; proteins; temperature; thermodynamics

The goal of this research program is to elucidate the physical mechanism of genetic control processes mediated by the interactions of proteins at specific sites on DNA. Components of the program include: (a) development of new techniques for the study of site- specific protein-DNA interactions, (b) theoretical work on the thermodynamic of coupled individual-site binding reactions in multi-site gene control systems, (c) experimental studies of functional energetics in specific gene control systems, (d) correlation of the energetic properties with structural features of the interacting molecules, (e) development and testing of physical-chemical models for gene control systems. We plan to extend our previous studies on the repressor-operator control system of bacteriophage lambda, which is a prototype for a large family of cooperative multi-site gene control systems. Using the Quantitative DNase Footprint Titration technique that we have recently developed, we will carry out a detailed study of the effects of temperature, pH, and ionic interactions on the site- specific energetics (including cooperativity effects) of cI and cro repressor binding to the DNA operator sites. The results will provide new insights into the detailed roles of proton binding, cation binding, and the various non-covalent forces responsible for the regulatory interactions. We will study the biological role of cooperative interactions between operator-bound repressors by in vivo and in vitro studies of mutant repressors that have altered cooperativity, using a physical-chemical model of gene control. We also plan further development of theory and techniques for understanding site-specific protein-DNA interactions.

这项研究计划的目标是阐明 相互作用介导的遗传控制过程的机制 DNA上特定位置的蛋白质。计划的组成部分 包括：(A)研究遗址的新技术-- 特定蛋白质-DNA相互作用，(B)关于蛋白质-DNA相互作用的理论工作 单位偶合结合反应的热力学 多位点基因控制系统，(C)实验研究 特定基因控制系统中的功能能量学，(D) 能量性质与结构特征的相关性 相互作用的分子，(E)开发和测试 基因控制系统的物理化学模型。 我们计划扩展我们以前对抑制算子的研究。 噬菌体Lambda的控制系统，这是一个原型 协作性多位点基因控制系统大家族。 使用定量DNA酶足迹滴定技术，我们 最近开发的，我们将对其进行详细研究 温度、pH和离子相互作用对位置的影响- 词与词的特定能量学(包括协同效应) 抑制物结合到DNA操纵子位点。结果将会是 提供了对质子结合的详细作用的新见解， 阳离子结合和各种非共价力负责 监管方面的相互作用。 我们将研究合作互动的生物学作用 操纵子结合的抑制子的体内和体外研究 改变了协作性的突变抑制子，使用 基因控制的物理化学模型。 我们还计划进一步发展理论和技术，以 了解特定部位的蛋白质-DNA相互作用。