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HEMOGLOBIN STRUCTURE-FUNCTION & BLOOD SUBSTITUTE DESIGN

血红蛋白结构-功能

基本信息

批准号：
2609319
负责人：
Gary K Ackers
金额：
$ 115.34万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-12-01 至 1999-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2609319
关键词：
blood /plasma substitute hemoglobin hemoprotein structure protein structure function

项目摘要

The central goal is to develop a better understanding of the factors and elements which control functional behavior of the human hemoglobin molecule. The approach that will be employed is to identify the roles of amino acid side chains in the molecule's normal functioning by residue substitution at specifically targeted sites coupled with extensive structural and functional characterization of the resulting hemoglobin molecules. This Program Project brings together seven research groups that have complementary areas of expertise and a common focus of interest on mechanisms of hemoglobin function and structure. In order to achieve this concentrated effort, it has been necessary to bring together individuals from a number of different institutions. (We know of no single institution with a comparable level of interest and expertise in this field.) This group has a proven track record of productive collaboration during the previous period of this grant. Other strengths include: a) efficiently functioning core facilities that have been developed under the current funding of this Program Project for production of large amounts of genetically engineered hemoglobin variants. b) Facilities and expertise for detailed, systematic characterization of structural (i.e., x-ray, spectroscopic, and theoretical) and functional (kinetic and thermodynamic) properties of hemoglobin. Work in the different laboratories will be coordinated through planned collaborations, exchanges of materials and personnel, and quarterly meetings. Results will be synthesized into a predictive model of the hemoglobin mechanism. This new level of understanding may permit the rational design of structurally-modified hemoglobins with desired properties, including potential red cell substitute materials.

中心目标是更好地理解这些因素和

项目成果

期刊论文数量（49）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Chloride acts as a novel negative heterotropic effector of hemoglobin Rothschild (beta 37 Trp-->Arg) in solution.

氯化物在溶液中充当罗斯柴尔德血红蛋白（β 37 Trp-->Arg）的新型负异向效应子。

DOI：
10.1021/bi00180a033
发表时间：
1994
期刊：
Biochemistry
影响因子：
2.9
作者：
Kelly,RM;Hui,HL;Noble,RW
通讯作者：
Noble,RW

Structural and dynamic properties of the homodimeric hemoglobin from Scapharca inaequivalvis Thr-72-->Ile mutant: molecular dynamics simulation, low temperature visible absorption spectroscopy, and resonance Raman spectroscopy studies.

来自 Scapharca inaequivalvis Thr-72-->Ile 突变体的同二聚血红蛋白的结构和动态特性：分子动力学模拟、低温可见吸收光谱和共振拉曼光谱研究。

DOI：
10.1016/s0006-3495(98)77693-3
发表时间：
1998
期刊：
Biophysical journal
影响因子：
3.4
作者：
Falconi,M;Desideri,A;Cupane,A;Leone,M;Ciccotti,G;Peterson,ES;Friedman,JM;Gambacurta,A;Ascoli,F
通讯作者：
Ascoli,F

Assignment and analysis of fluorine nuclear magnetic resonance spectra of 4-fluorotryptophan myoglobins and hemoglobins.

4-氟色氨酸肌红蛋白和血红蛋白的氟核磁共振谱的归属和分析。