VITAMIN A IN GROWTH AND DEVELOPMENT

生长和发育中的维生素 A

• 批准号: 3311060
• 负责人: FRANK CHYTIL
• 金额: $ 16.91万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3311060
• 关键词: RNA; all trans retinol; analytical method; autoradiography; cell differentiation; cell nucleus; developmental nutrition; electron microscopy; gene expression; genetic transcription; glucocorticoids; growth /development; histochemistry /cytochemistry; immunochemistry; intestinal mucosa; liver; lung; mature animal; molecular pathology; newborn animals; nutrition related tag; postnatal growth disorder; radioimmunoassay; radiotracer; retinoate; testis; tissue /cell culture; vitamin A deficiency; vitamin metabolism

The objective of proposed in vivo as well as in vitro nutritional and biochemical research is to continue to define the mechanisms(s) of action of the important micronutrient vitamin A at the molecular level in organs other than the eye. The participation in the mode of vitamin action of two distinct specific intracellular cytoplasmic binding proteins for retionl (vitamin A alcohol) and for retinoic acid (vitamin A acid) purified to homogeneity from rat liver and testis will receive particular attention. In vitro studies are proposed how retinol and retinoic acid bound to their respective binding proteins interact with the cell nucleus changing thereby the genomic expression underlying cell differentiation. The in vivo studies are directed towards the elucidation of factor which regulate the levels of these binding proteins in various organs in adult and in neonatal rats. To this end sensitive radioimmunoassys developed for both binding proteins will be utilized.

所提出的体内以及体外营养和 生化研究将继续确定作用机制 器官中重要微量营养素维生素 A 在分子水平上的研究 除了眼睛之外。 两种维生素参与维生素作用的方式 不同的细胞内特异性结合蛋白 （维生素A醇）和视黄酸（维生素A酸）纯化至 大鼠肝脏和睾丸的同质性将受到特别关注。 体外研究提出了视黄醇和视黄酸如何与其结合 各自的结合蛋白与细胞核相互作用，从而改变 细胞分化的基因组表达。 体内 研究的目的是阐明调节因素 这些结合蛋白在成人和新生儿各器官中的水平 老鼠。 为此，开发了敏感的放射免疫分析系统，用于结合 蛋白质将被利用。