BACTERIAL MINICELL MUTANTS

该项目的长期目标是了解 细菌细胞用来将分裂部位放置在其 在细胞内的适当位置。为了实现这一目标， 细菌微细胞座位(MinB)将被研究，因为突变 这一基因座导致隔膜位置异常。 这些研究是基于最近的研究表明，mini B基因座 影响分裂过程的几个基因的密码。这个 研究将结合遗传学、生物化学和 免疫电子显微镜将minB基因产物定位于 细胞，以确定它们之间的相互作用以及与 细胞分裂机制中的其他蛋白质成分，以探查 其中一种蛋白质的可能的酶功能，以及 研究minB系统与另一个基因的相互作用 可能对细胞分裂过程产生影响的产物(DicB) 由minB系统介导。

The long-range objective of this project is to understand the mechanism used by bacterial cells to place the division site at its proper location within the cell. To approach this goal, the bacterial minicell locus (minB) will be studied since mutation of this locus results in aberrant placement of the division septum. The studies are based on recent work showing that the miniB locus codes for several genes that affect the division process. The studies will use a combination of genetics, biochemistry and immunoelectronmicroscopy to localize the minB gene products within the cell, to identify their interactions with each other and with other protein components of the cell division machinery, to probe for possible enzymatic functions of one of the proteins, and to examine the interaction of the minB system with another gene product (dicB) whose effect on the cell division process is likely to be mediated by the minB system.