LONG-TERM ORGAN PRESERVATION FOR TRANPLANTATION

这项研究的目的是开发一种安全可靠的技术 用于长期保存器官以供移植。 器官移植已被证明在治疗先天性心脏病方面取得了成功。 终末期器官衰竭患者。本程序的适用范围 由于缺乏合适的捐献器官而受到严重限制。安全 心脏和肺的保存时间仍然被限制在4-6小时。 时间如此之短的方法有三个主要缺点： (1)它限制了获得移植物的地理区域。(2)它 限制组织相容性测试的驯服，以及(3)它引发 组织保护不足的可能性。先进的技术 心脏、肺和心脏等重要器官的保存技术 肝脏是极其重要的，因为长期的替代方法 人工支持是不存在的。 我们已经开发和研究了一种新的长期自灌流技术 器官保存。对动物进行麻醉和人工处理 通风，心、肺、肝、胰腺、十二指肠和两肾 在心脏灌流的同时被整体移除，并被 肺部。呼吸器用于通风。新鲜血液， 电解质、抗生素和营养素通过静脉滴注进行输注。 不需要抗凝剂或变力药物。这是一种自我- 包含和自我调节的系统，只需要很少的干预。 心脏将血液输送到所有器官。肺为血液提供氧气。 肝脏维持其生物学功能。电解液和水 平衡被维持，代谢废物被肾脏排出。 这种准备保留了器官的自然解剖联系。 在没有外来物质的帮助下，能量和衬底 器官之间的交换被保存下来。在一项先导研究中，存活率 驯服时间为41~60小时，血流动力学和脏器稳定 功能。在这项建议中，我们打算研究 血小板聚集与组织存活时间的关系研究 在底物摄取和器官功能之间；研究器官功能和 不同温度环境下的存活时间；移植心脏 保存24小时后；12-24小时后移植心脏 自灌流保存时间加4-6小时低温保存时间 储藏室。以前还没有做过类似的研究。这些研究将 获得关于体外器官功能和存活的新知识 长期自动灌流，并为可能的 这项技术的临床应用。

The objective of this study is to develop a safe and reliable technique for long-term organ preservation for transplantation. Organ transplantations have proved successful in the treatment of patients with end-stage organ failure. Application of these procedures is severely Limited by the lack of suitable donor organs. Safe preservation time for the heart and lungs Is still limited to 4-6 hours. Methods with such a short period of time have three main disadvantages: (1) It limits the geographic area from which to obtain grafts. (2) It limits the tame for histocompatibility tests and (3) It raises the possibility of inadequate tissue protection. Advancements in preservation techniques for vital organs such as the heart, lungs and liver are extremely important since alternative methods of long-term artificial support do not exist. We have developed and studied a new autoperfusion technique for long-term organ preservation. With the animals are anesthetized and artificially ventilated, the heart, lungs, liver, pancreas, duodenum and both kidneys are removed en bloc while being perfused by the heart and oxygenated by the lungs. A respirator is used for ventilation. Fresh blood, electrolytes, antibiotics and nutrients are infused by intravenous drip. No anticoagulants or inotropic drugs are necessary. This is a self- contained and self-adjusted system and requires very little intervention. The heart pumps blood to all the organs. The lungs oxygenate the blood. The liver maintains its biological functions. Electrolyte and water balances are maintained and metabolic wastes are removed by the kidneys. This preparation preserves the natural anatomic connections of the organs without assistance of foreign material, and the energy and substrate exchanges between organs are preserved. In a pilot study, the survival tame ranged from 41 to 60 hours with stable hemodynamics and organ function. In this proposal, we intend to study the relationship between platelet aggregation and tissue survival time; study the relationship between substrate uptake and organ function; study organ function and survival time under different temperature settings; transplant the heart after 24 hours of preservation; and transplant the heart after 12-24 hours of autoperfusion preservation plus 4-6 hours of hypothermic storage. Similar studies have not been done before. These studies will gain new knowledge of ex vivo organ function and survival during long-term autoperfusion and provide further evaluation for possible clinical application of this technique.