BIOCHEMICAL APPROACHES TO THE CELL DIVISION PROBLEM

细胞分裂问题的生物化学方法

• 批准号: 2180579
• 负责人: JAMES T PARK
• 金额: $ 16.49万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2180579
• 关键词: Escherichia coli; aminoacyltransferase; bacterial genetics; bacterial proteins; binding proteins; cell cycle; crosslink; cytogenetics; developmental genetics; enzyme complex; enzyme mechanism; galactosyltransferases; gene expression; gram negative bacteria; high performance liquid chromatography; protein biosynthesis; synchronous cell division; temperature sensitive mutant

The long term objective of this research is to elucidate the biochemical steps required for septation and cell separation in a gram negative organism such as Escherichia coli. More specifically, the work will be concerned with identifying and characterizing the function of the proteins involved in formation and separation of new polar caps in the septum. Presumably a number of enzymes are needed for de novo synthesis of the polar caps but only one penicillin-binding protein 3, has been identified. Yet at least another twelve gene products are essential for septation. The research seeks to identify the role of these essential proteins. The specific aims are to: 1. Identify the chemical structures and enzymatic reactions that are unique to septation. and 2. Characterize multiprotein complexes involved in septation. The latter objective involves isolation and characterization of extragenic suppressors of cell division mutants, isolation of protein complexes by appropriate physical methods, and identification of the enzymatic activities and protein components therein. Obviously only a pioneering start can be made toward achieving the final objectives but understanding cell division is of such fundamental importance that it seems appropriate that someone begins a more direct attack to learn something about the role of the twelve or more gene products required for septation.

本研究的长期目标是阐明