INSTRUMENTATION FOR ANALYSIS OF HIGH MASS BIOMOLECULES

高质量生物分子分析仪器

• 批准号: 3304824
• 负责人: SCOTT A MCLUCKEY
• 金额: $ 22.05万
• 依托单位: LOCKHEED MARTIN ENERGY SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3304824
• 关键词: biomedical equipment development; chemical structure function; intermolecular interaction; ionization; macromolecule; mass spectrometry; molecular weight

The long-term objective of this proposal is to establish the foundation for a relatively small, inexpensive, but very powerful instrument for obtaining structural information from and molecular weights of high mass biomolecules. Specifically, the instrument will couple electrospray ionization with a quadrupole ion trap mass spectrometer. Electrospray ionization has been shown to be capable of producing highly charged ions from large biomolecules (MW > 104 Da). A quadrupole ion trap can be used to manipulate these ions both to provide molecular weights and to provide structural information. This work is devoted to optimizing the interface between the electrospray on source and the mass analyzer, exploring and optimizing instrumental techniques for mass/charge measurement, and performing fundamental research on the reactivities of the highly charged biomolecules in the ion trap environment. The latter work is designed to maximize both sensitivity and the amount of structural information obtained. This work will benefit the biomedical community by providing relatively low-cost means for making accurate molecular weight determinations and for obtaining structural information from a wide range of high mass molecules. The proposed instrument, due to its small size and low cost, would be much more accessible to researchers in the biomedical community than the much larger and more expensive instrumentation presently available.

该提案的长期目标是为以下方面奠定基础： 一种相对较小、廉价但功能非常强大的工具，用于获取 来自高质量的结构信息和分子量 生物分子。 具体来说，该仪器将耦合电喷雾 使用四极离子阱质谱仪进行电离。 电喷雾 电离已被证明能够产生高电荷离子 来自大生物分子（MW > 104 Da）。 可以使用四极离子阱 操纵这些离子既提供分子量又提供 结构信息。 这项工作致力于优化界面 在源电喷雾和质量分析器之间，探索和 优化质量/电荷测量的仪器技术，以及 对高电荷反应性进行基础研究 离子阱环境中的生物分子。 后者的工作旨在 最大化灵敏度和结构信息量 获得。 这项工作将通过提供使生物医学界受益 相对低成本的精确分子量测定方法 测定并从广泛的范围内获取结构信息 的高质量分子。 所提议的仪器由于其尺寸小且 成本低，生物医学研究人员更容易获得 比目前更大、更昂贵的仪器更受欢迎 可用的。