INSTRUMENTATION FOR ANALYSIS OF HIGH MASS BIOMOLECULES

高质量生物分子分析仪器

• 批准号: 2415148
• 负责人: SCOTT A MCLUCKEY
• 金额: $ 23.26万
• 依托单位: LOCKHEED MARTIN ENERGY RESEARCH CORP
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2415148
• 关键词: biomedical equipment development; electrospray ionization mass spectrometry; oligonucleotides

DESCRIPTION: The objective of this work is to advance the state-of-the- art in electrospray/ion trap mass spectrometry of biomolecules by improving the analytical figures of merit of the instrumentation as well as by developing new experiments to gain information on high mass biologically relevant ions. The quadrupole ion trap is a remarkably powerful tool for the manipulation and mass/charge analysis of biologically-derived ions. Furthermore, it is far less expensive than other forms of instrumentation capable of performing tandem mass spectrometric experiments. The latter characteristic makes the ion trap potentially accessible to a wide range of researchers in the biomedical field. This work seeks to achieve ultra-low level analysis/detection of high mass biomolecules (low attomole levels), without preconcentration, in common analytical scenarios such as on-line capillary electrophoresis and flow injection analysis. Particular emphasis will be placed on the use of non-destructive ion detection and re-measurement within the context of multi-stage mass spectrometry experiments. The ability to store ions will be used to develop new applications for ion/molecule reactions and to explore the chemistry associated with reactions of multiply-charged biopolymers with oppositely charged ions. The ion/molecule reaction applications include a technique to increase ion currents derived from multiply-charged biopolymers by several orders of magnitude via proton transfer reactions and an approach to dealing with electrospray of mixtures involving tandem mass spectrometry and proton transfer reactions. Ion/ion reaction studies will focus on both proton transfer and electron transfer with particular attention paid to the chemistry of the odd-electron species formed via electron transfer reactions. In all cases, emphasis will be placed on experiments that can provide new information or that can enhance the performance of the ion trap over current methodology.

描述：这项工作的目标是推进现状 生物分子电喷雾/离子阱质谱分析技术 还提高了仪器的分析品质因数 例如通过开发新的实验来获取高质量的信息 生物学相关的离子。 四极杆离子阱是一个非常强大的工具 生物衍生离子的操纵和质量/电荷分析。 此外，它比其他形式的成本要低得多 能够进行串联质谱分析的仪器 实验。 后一个特性使得离子阱具有潜在的潜力 可供生物医学领域的广泛研究人员使用。 这 工作旨在实现高质量的超低水平分析/检测 生物分子（低阿托摩尔水平），无需预浓缩，共同点 分析场景，例如在线毛细管电泳和流式细胞术 注射分析。 将特别强调使用 无损离子检测和重新测量 多阶段质谱实验。 储存离子的能力 将用于开发离子/分子反应的新应用以及 探索与多电荷反应相关的化学 带有相反电荷离子的生物聚合物。 离子/分子反应 应用包括增加离子电流的技术 通过质子将多电荷生物聚合物提高几个数量级 转移反应和处理电喷雾的方法 涉及串联质谱和质子转移的混合物 反应。离子/离子反应研究将集中于质子转移 和电子转移，特别关注化学 通过电子转移反应形成的奇电子物种。 在 在所有情况下，重点将放在能够提供新的实验上 信息或可以增强离子阱的性能 当前的方法。