BIO-ORGANIC PHOTOCHEMISTRY

生物有机光化学

• 批准号: 3306176
• 负责人: MICHAEL C PIRRUNG
• 金额: $ 20.02万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3306176
• 关键词: biotin; chemical binding; chemical group; chemical synthesis; fluorescent dye /probe; method development; molecular site; nucleic acid chemical synthesis; photoactivation; photochemistry

The long term goal of this research is to provide biomedical researchers with a selection of photoreactive functionalities that can be incorporated into bioactive molecules. These groups will permit the cross-linking of macromolecules and small molecules so as to identify sites of interaction and the preparation of bioactive molecules in specific locations. The groups to be developed are designed to be superior to currently used groups regarding their efficiency of reaction, quantum yield, wavelength of activation, by-products, and ease of incorporation into biomolecules. The project also aims to develop methods whereby such groups can be used to localize different biological components for assembly into large ensembles with special functions or as model systems. The specific aims of this project are to develop new photoremovable silyl groups, nitrobenzyl groups, and phenylglyoxyl groups. These photoremovable groups will be used in "caging" (protecting from reaction by a photoremovable group) serine and enols. Caging is useful in locating an inactive effector molecule and rapidly activating it to observe its biological effects or chemical reactivity. Photoremovable groups will be used in the synthesis of DNA sequences at specific locations on a surface. Photoactivatable surfaces that can bind cells in irradiated zones will also be developed. The key to the methods to be developed in the project is the ability to spatially direct light and to detect binding by fluorescence. The ability to synthesize DNA in defined locations will permit a number of new applications of nucleic acid hybridization to be developed, including diagnostics, clone mapping, and DNA sequencing.

这项研究的长期目标是为生物医学研究人员提供 具有一系列光反应功能，可以 并入生物活性分子。 这些团体将允许 通过交联大分子和小分子来识别 相互作用位点和生物活性分子的制备 具体地点。 待开发的组被设计为 就反应效率而言优于目前使用的基团， 量子产率、激活波长、副产物和易于 并入生物分子。 该项目还旨在开发 这些方法可以用来定位不同的生物 用于组装成具有特殊功能的大型整体的组件或作为 模型系统。 该项目的具体目标是开发新的光可去除硅基 基团、硝基苄基和苯基乙氧基。 这些 光可去除基团将用于“笼蔽”（防止反应） 由光可去除基团）丝氨酸和烯醇。 笼养适用于 定位不活跃的效应分子并快速激活它 观察其生物效应或化学反应性。可光去除 基团将被用于在特定的 DNA 序列的合成 表面上的位置。 可结合细胞的光活化表面 还将开发辐射区。 方法的关键在于 该项目开发的是空间引导光和 通过荧光检测结合。 以规定的方式合成DNA的能力 这些地点将允许核酸的许多新应用 待开发的杂交技术，包括诊断、克隆作图和 DNA 测序。