GENE REGULATION IN TERATOCARCINOMA AND EMBRYONIC CELLS

畸胎瘤和胚胎细胞中的基因调控

• 批准号: 3314897
• 负责人: SIDNEY STRICKLAND
• 金额: $ 16.37万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3314897
• 关键词: carcinoma; cell differentiation; early embryonic stage; egg /ovum; embryo /fetus cell /tissue; gene expression; genetic enhancer element; genetic manipulation; genetic regulation; genetic transcription; genetically modified animals; in situ hybridization; laboratory mouse; mammalian embryology; molecular cloning; neoplasm /cancer genetics; neoplastic cell; nucleic acid sequence; plasminogen activator; teratoma; tissue /cell culture

We propose the following areas of investigation: 1. To study the determinants that regulate the transcription of the tissue plasminogen activator (t-PA) gene using three experimental murine systems: a) teratocarcinoma stem cells in various stages of differentiation; b) extracts of tissues that variably express the t-PA gene; and c) growing oocytes. 2. To investigate the role of t-PA in mouse embryonic development and normal physiology by: a) examining spatial and temporal aspects of t-PA mRNA expression in the embryo and adult by in situ analysis; b) in conjunction with Aim 1, determining which regions of the t-PA promoter are instrumental in tissue-specific expression by analyzing transgenic mice containing t-PA promoter/reporter gene constructs; and C) investigating the consequences of disruption of one or both copies of the t-PA gene in mice using the techniques of homologous recombination. 3. To characterize a new type of plasminogen activator (PA) produced by a Saturnidae moth caterpillar.

我们建议进行以下调查： 1.研究调节组织转录的决定因素 纤溶酶原激活物（t-PA）基因，使用三种实验鼠系统： a）处于不同分化阶段的畸胎癌干细胞; B） 表达t-PA基因的组织的提取物;和c）生长 卵母细胞 2.探讨t-PA在小鼠胚胎发育中的作用， 正常生理学通过：a）检查t-PA的空间和时间方面 通过原位分析在胚胎和成体中的mRNA表达; B）在胚胎和成体中的mRNA表达。 与目标1结合，确定t-PA启动子的哪些区域是 通过分析转基因小鼠有助于组织特异性表达 含有t-PA启动子/报告基因构建体;和C）研究 小鼠中t-PA基因的一个或两个拷贝破坏的后果 使用同源重组技术。 3.目的：研究一种新型纤溶酶原激活剂（PA）的特性， 土星蛾毛虫。