PROGESTIN AND RELAXIN EFFECTS ON ENDOMETRIAL PROLACTIN

最近的研究表明，孕激素和松弛素能协同诱导 人催乳素(PRL)等细胞蛋白的产生 子宫内膜间质细胞。孕激素和松弛的协同作用 催乳素的诱导作用与生长发育密切相关 妊娠时子宫内膜间质细胞的蜕膜化 在怀孕期间。这个项目的长期目标是调查 不同时期个体激素在催乳素诱导中的作用 子宫内膜间质细胞的发育。 该项目建议研究孕激素的作用机制。 和松弛素对不同水平的PRL基因表达和 人子宫内膜间质细胞在长期培养中的发育 系统。(1)PRL合成率、PRLmRNA的稳态水平、 PRLmRNA的降解率和PRLmRNA的转录速率， 将在初始阶段和持续的PRL期间进行调查 生产期。(2)子宫内膜间质细胞的发育 黄体酮和松弛素对体外的影响将通过 催乳素在间质细胞中的诱导模式比较 蜕膜，一种完全分化的子宫内膜；通过研究DNA 合成、总DNA和蛋白质含量。(3)第二信使(S) 松弛素与传递血管紧张素转换酶信号的跨膜信号通路 松弛素用来激活PRL基因表达的研究将在 孕激素刺激的子宫内膜间质细胞和蜕膜细胞。 本课题提供了一个研究PRL基因表达的系统。 子宫内膜的分化，一个独特的不同的生物系统 来自脑下垂体系统。这项提议将证明 荷尔蒙靶标组织(子宫内膜)转变为 自分泌系统(蜕膜)。了解松弛素的作用 内源性激素对蜕膜成形术成功率的影响 植入。它还将为生育提供新的药理途径。 规范和开发新的避孕药，预防 以及生殖功能障碍和肿瘤转化的治疗 子宫内膜。

Recent studies show that progestin and relaxin synergistically induce the production of prolactin (PRL) and other cellular proteins in human endometrial stromal cells. The concerted effects of progestin and relaxin on the induction of prolactin are closely associated with the growth and decidualization of the endometrial stromal cells at the time of conception and during pregnancy. The long term goal of this project is to investigate the role of individual hormones on the induction of PRL at different stages of the development of endometrial stromal cells. The project proposes to investigate the mechanism of action of progestin and relaxin on the various levels of PRL gene expression and the development of human endometrial stromal cells in a long-term culture system. (1) The rate of PRL synthesis, steady-state levels of PRLmRNA, the rate of degradation of PRLmRNA, and the rate of transcription of PRLmRNA, will be investigated at the initial phase and during the continuing PRL production period. (2) The development of endometrial stromal cells under the influence of progestin and relaxin in vitro will be studied by comparing the induction pattern of PRL in the stromal cells to that in the decidua, a fully differentiated endometrium; and by investigating DNA synthesis, total DNA and protein contents. (3) The second messenger(s) of relaxin and the transmembrane signal pathways which transmit the signal of relaxin to activate the PRL gene expression will be investigated in progestin-primed endometrial stromal cells and in decidual cells. This project provides a system to study the PRL gene expression during the differentiation of the endometrium, a biological system uniquely different from the pituitary system. This proposal will demonstrate the transformation of a hormone target tissue (the endometrium) into an autocrine system (the decidua). Understanding the role of relaxin and endogenous hormone on decidualization will improve the successful rate of implantation. It will also offer new pharmacological avenues to fertility regulation and the development of new contraceptive agents, the prevention and treatment of reproductive dysfunction and neoplastic transformation of the endometrium.