NEUROENDOCRINE REGULATION OF OVARIAN CYCLICITY

季节性繁殖中卵巢周期性的神经内分泌调节 物种是这个项目的主题。将进行研究，以 识别与光周期、松果体、下丘脑和 类固醇对促黄体生成素搏动性分泌的调节及其对雌二醇的反应 母羊的负反馈。我们将特别强调 关于这些信号的模式化对产生的重要性 回应。下丘脑促性腺激素释放激素和松果体褪黑素的信号模式 将特别引起人们的兴趣，并将使用便携式背包进行研究 输液系统。立体定位下丘脑的刀伤和 促性腺激素释放激素在绵羊脑神经元的免疫细胞化学定位 用于研究光周期和类固醇的神经加工 调节脉动性黄体生成素分泌的信号。放射免疫分析将是 用于测定血清促黄体生成素、卵泡刺激素、促性腺激素释放激素、褪黑素、雌二醇和 孕激素。预计这些发现将带来新的洞察力 关于卵巢周期性和季节性繁殖，这是一个自然的过程 可逆的生育能力。

The neuroendocrine regulation of ovarian cyclicity in a seasonally breeding species is the theme of this project. Studies will be performed to identify signals pertinent to photoperiodic, pineal, hypothalamic and steroidal regulation of pulsatile LH secretion and response to estradiol negative feedback in the female sheep. Particularl emphasis will be placed on the importance of patterning of these signals to generation of responses. The patterns of hypothalamic GnRH and pineal melatonin signals will be of special interest and will be studied using a portable back-pack infusion system. Stereotaxically placed knife-cuts in the hypothalamus and immunocytochemical localization of GnRH in neurons of the sheep brain will be used to examine the neural processing of photoperiodic and steroidal signals which regulate pulsatile LH secretion. Radioimmunoassays will be used to measure serum LH, FSH, GnRH, melatonin, estradiol and progesterone. It is expected that these findings will lead to new insight concerning ovarian cyclicity and seasonal breeding, an natural process of reversable fertility.