A CELL LINEAGE STUDY OF AVIAN SOMITE FORMATION

鸟类体节形成的细胞谱系研究

• 批准号: 3314396
• 负责人: DAVID S. PACKARD
• 金额: $ 6.63万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-02-01 至 1987-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3314396
• 关键词: cinemicrography; congenital skeletal disorder; dissection; embryo /fetus culture; mesoderm; spine; transplantation

The work proposed in this application is intended to increase our knowledge of how the veterbrate somite pattern is generated and maintained. Specifically it is proposed to use embryo culture and grafting techniques to exchange portions of the unsegmented somite mesoderm between embryos of the chicken and the Japanese quail. These exchanges will be carried out in such a way as to create disharmonies between the somite pattern specified by the host embryo and the somite pattern that would have been formed by the grafted mesoderm if it had been left in place. Through the use of histological analysis and time-lapse cinemicrography, it will be determined if the host embryos have the ability to regulate the number and time of formation of the graft somites. The information derived from this study is expected to aid in our eventual understanding of the morphogenesis and the teratogenesis of the vertebral column.

本申请中提出的工作旨在增加我们的知识 脊椎动物的体节模式是如何产生和维持的。 具体来说就是 建议采用胚胎培养和嫁接技术来交换部分 鸡胚胎和鸡胚胎之间未分节的体节中胚层 日本鹌鹑。 这些交流将以创造 宿主胚胎指定的体节模式与宿主胚胎指定的体节模式之间的不协调 如果嫁接的中胚层有的话，它会形成体节模式 被留在原地。 通过使用组织学分析和延时 电影显微摄影，将确定宿主胚胎是否有能力 调节移植体节形成的数量和时间。 信息 这项研究的结果预计将有助于我们最终理解 脊柱的形态发生和畸形发生。