A CELL LINEAGE STUDY OF AVIAN SOMITE FORMATION

本申请中提出的工作旨在增加我们对 老脊椎动物体节图案是如何产生和维持的。具体来说就是它 建议使用胚胎培养和嫁接技术来交换部分 鸡胚胎与鸡胚间未分段的体节中胚层 日本鹌鹑。这些交流将以这样一种方式进行，以创造 寄主胚胎所指定的体节模式与 如果有，移植的中胚层就会形成体节图案 一直留在原地。通过使用组织学分析和时间推移 电影显微摄影，将确定宿主胚胎是否有能力 调节嫁接体节形成的数量和时间。这些信息 从这项研究中得出的结论有望帮助我们最终理解 脊柱的形态发生和畸形发生。

The work proposed in this application is intended to increase our knowledge of how the veterbrate somite pattern is generated and maintained. Specifically it is proposed to use embryo culture and grafting techniques to exchange portions of the unsegmented somite mesoderm between embryos of the chicken and the Japanese quail. These exchanges will be carried out in such a way as to create disharmonies between the somite pattern specified by the host embryo and the somite pattern that would have been formed by the grafted mesoderm if it had been left in place. Through the use of histological analysis and time-lapse cinemicrography, it will be determined if the host embryos have the ability to regulate the number and time of formation of the graft somites. The information derived from this study is expected to aid in our eventual understanding of the morphogenesis and the teratogenesis of the vertebral column.