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A CELL LINEAGE STUDY OF AVIAN SOMITE FORMATION

鸟类体节形成的细胞谱系研究

基本信息

批准号：
3314397
负责人：
DAVID S. PACKARD
金额：
$ 6.57万
依托单位：
UPSTATE MEDICAL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-02-01 至 1987-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3314397
关键词：
cinemicrography congenital skeletal disorder dissection embryo /fetus culture mesoderm spine transplantation

项目摘要

The work proposed in this application is intended to increase our knowledge of how the veterbrate somite pattern is generated and maintained. Specifically it is proposed to use embryo culture and grafting techniques to exchange portions of the unsegmented somite mesoderm between embryos of the chicken and the Japanese quail. These exchanges will be carried out in such a way as to create disharmonies between the somite pattern specified by the host embryo and the somite pattern that would have been formed by the grafted mesoderm if it had been left in place. Through the use of histological analysis and time-lapse cinemicrography, it will be determined if the host embryos have the ability to regulate the number and time of formation of the graft somites. The information derived from this study is expected to aid in our eventual understanding of the morphogenesis and the teratogenesis of the vertebral column.

本申请中提出的工作旨在增加我们的知识脊椎动物的体节模式是如何产生和维持的。具体来说就是建议采用胚胎培养和嫁接技术来交换部分鸡胚胎和鸡胚胎之间未分节的体节中胚层日本鹌鹑。这些交流将以创造宿主胚胎指定的体节模式与宿主胚胎指定的体节模式之间的不协调如果嫁接的中胚层有的话，它会形成体节模式被留在原地。通过使用组织学分析和延时电影显微摄影，将确定宿主胚胎是否有能力调节移植体节形成的数量和时间。信息这项研究的结果预计将有助于我们最终理解脊柱的形态发生和畸形发生。