A STUDY OF MEMBRANES ISOLATED FROM GAMETES

翻译后摘要：细胞内[H +]（pHi）和[Ca++]的变化是重要的， 海胆配子的激活 这项工作的目标是描述 这些离子运动的机制在孤立的水平， 膜。 Na+依赖性的pHi增加导致启动 将在三个水平上研究精子活力：完整精子、分离精子 精子头部和尾部，以及由其衍生的膜制品。 将开发方法来监测这些细胞中的膜电位和pHi。 准备工作 离子输运性质的可能差异， 精子头部和尾部与其功能（顶体反应）相关 和运动性）进行检查。 质膜将被 和Na+依赖的H +转运活性 研究了 将努力查明下列因素： 在受精后激活这种活性。 酸性颗粒 在合子中出现的与pHi升高同时出现的蛋白质将被分离， 特征，并阐明其功能。 研究还建议， 制备完整的顶体颗粒及其膜。 这些膜 将被表征，它们的离子传输特性研究和努力 将其用作研究膜融合发生的体外系统 在顶体反应和受精过程中。

Abstract: Intracellular [H +] (pHi) and [Ca++] changes are important in the activation of sea urchin gametes. The goal of this work is to describe the mechanisms of these ionic movements at the level of isolated membranes. The Na+ dependent increase of pHi responsible for initiating sperm motility will be studied at three levels: the intact sperm, isolated sperm heads and tails, and membrane preparations derived from them. Methods will be developed to monitor membrane potential and pHi in these preparations. The possible differences in ion transport properties in sperm heads and tails correlating with their functions (acrosome reaction and motility respectively) will be examined. Plasma membranes will be prepared from eggs and zygotes and the Na+ dependent H + transport activity studied. Efforts will be made to identify the factors involved in activating this activity after fertilization. The acidic granules appearing in zygotes concomitant with the rise of pHi will be isolated and characterized, and their function elucidated. Research is also proposed to prepare intact acrosomal granules and their membranes. These membranes will be characterized, their ion transport properties studied and efforts made to use them as an in vitro system for studying membrane fusion occurs during the acrosome reaction and fertilization.