FETAL SKIN BIOLOGY

结构、生化和功能的多学科研究 计划开发过程中人类皮肤的特性。 正常样本 胚胎和胎儿皮肤将用于建立组织学和 表皮、真皮和羊水的超微结构特征 处于发育进展阶段的细胞群。 这些数据将 作为所有其他项目的共同点；平行样本 用于形态学研究的相同胚胎和胎儿的皮肤 将用于： 真皮-表皮半桥粒的定量 表皮的连接、生化测定和免疫定位 蛋白质丝聚蛋白和角蛋白，特定的生化鉴定 表皮、皮下脂肪和羊水中的脂质类别， 评估穿过表皮的运输、生化鉴定 和真皮中主要间质胶原蛋白的免疫定位， 以及真皮糖胺聚糖的鉴定和定量， 评估真皮胶原酶的合成和活性，在不同的条件下 发展阶段。 胎儿皮肤的活检和尸检样本 还将研究遗传性皮肤疾病的形态学 我们的组织发生了许多生化过程的改变 数量允许。 这些项目的数据预计将扩大我们的 了解发育过程中正常人类皮肤生物学，作为 控制，基线数据对于评估异常至关重要 有严重遗传风险的胎儿的皮肤结构和特性 外皮疾病，扩大产前诊断的潜力 皮肤疾病并扩展我们对自然史的理解 遗传性皮肤病进入产前期。

A multidisciplinary study of the structural, biochemical and functional properties of human skin during development is planned. Samples of normal embryonic and fetal skin will be used to establish histologic and ultrastructural characteristics of the epidermis, dermis and amniotic fluid cell populations at progressive stages of development. These data will serve as the common denominator of all other projects; parallel samples of skin from the same embryos and fetuses used for the morphologic studies will be used for: quantitation of hemidesmosomes at the dermal-epidermal junction, biochemical assay and immunolocalization of the epidermal proteins filaggrin and keratin, biochemical identification of specific classes of lipids in the epidermis, subcutaneous fat and amniotic fluid, evaluation of transport across the epidermis, biochemical identification and immunolocalization of the major interstitial collagens in the dermis, and identification and quantitation of dermal glycosaminoglycans, evaluation of synthesis and activity of dermal collagenase, at various stage of development. Biopsy and autopsy samples of skin from fetuses with inherited cutaneous disorders will also be studied for morphologic alterations and by as many of the biochemical procedures our tissue quantity allows. The data from these projects are expected to expand our understanding of normal human skin biology during development, serve as control, baseline data essential for evaluation of abnormalities in cutaneous structure and properties in fetuses at risk for severe inherited disorders of the integument, expand tee potential for prenatal diagnosis of skin disorders and extend our understanding of the natural history of genetic skin diseases into the antenatal period.