FETAL SKIN BIOLOGY

这是一个大型的多学科合作计划，其中 研究的主要目的是扩大我们对 正常人胚胎和胎儿结构、生化和功能 皮肤在发展过程中，并确定如何正常的属性是 由于遗传性皮肤病而在出生前发生改变。 研究计划于 三个类别：1）我们将继续描述 皮肤（表皮、真皮和真皮-表皮连接处）的发育 分化的各种结构和生化标志物的术语，2） 这些将提供对真皮-表皮相互作用的深入了解， 可能影响两种组织的分化和形态建成 3）与特定临床研究相关的。 第一类的研究包括对年龄相关的基因表达的测定， 外皮蛋白、EGF受体、细胞表面糖复合物和V型胶原 由表皮角质形成细胞和抗原和受体的朗格汉斯 细胞; VII型胶原与锚定的外观相关 DEJ处的原纤维;以及真皮中的弹性蛋白和GAG。 形态测定 计划对表皮细胞表面进行研究， 了解皮肤在早期运输过程中的可能作用 怀孕 早产儿皮肤的结构和生物化学 研究和计算机辅助重建将用于评估 皮肤神经/血管网络的发育。 细胞培养，器官 培养、人工基质培养和胎儿皮肤裸移植 将是用于评估真皮-表皮相互作用的系统， 组织重组实验。 临床研究的重点是 对胎儿和新生儿皮肤样本的评价 受遗传性皮肤病影响的成年患者的研究， 外胚层发育不良;“新鲜”羊膜的生化特征 液体细胞;子宫内活组织检查胎儿伤口愈合的研究;以及 检查暴露于异维A酸的胎儿和新生儿的组织 子宫（维生素A畸形学）。 已计划达成一项财团协议， 继续研究脂质的表达和生物合成（培养）， 胚胎、胎儿和早产儿皮肤。

This is a large multidisciplinary, collaborative program in which the primary objectives of the research are to expand our understanding of the structure, biochemistry and function of normal human embryonic and fetal skin during development, and to determine how the normal properties are altered antenatally by inherited skin disease. Studies are planned in three categories: 1) those in which we will continue to characterize the development of skin (epidermis, dermis and dermal-epidermal junction) in terms of various structural and biochemical markers of differentiation, 2) those which will provide insight into dermal-epidermal interactions that may influence the differentiation of the two tissues and the morphogenesis of the epidermis, and 3) those related to specific clinical studies. Studies in the first category include assays for age-related expression of involucrin, EGF-receptors, cell surface glycoconjugates and type V collagen by epidermal keratinocytes, and of antigens and receptors by Langerhans cells; of type VII collagen correlated with the appearance of anchoring fibrils at the DEJ; and of elastin and GAGs in the dermis. Morphometric studies of the epidermal cell surface are planned to increase our understanding of a possible role of the skin in transport during early gestation. The structure and biochemistry of premature skin will be studied and computer-assisted reconstruction will be used to evaluate the development of nerve/vessel networks of the skin. Cell culture, organ culture, artificial matrix cultures and grafts of fetal skin to the nude will be systems employed to evaluate dermal-epidermal interactions in tissue recombinant experiments. The clinical studies are focused on the evaluation of skin samples obtained at fetos-copy, and from newborns affected with inherited skin disease, studies of adult patients with ectodermal dysplasias; biochemical characterization of "fresh" amniotic fluid cells; studies of healing wounds from fetuses biopsied in utero; and examination of tissue from fetuses and newborns exposed to isotretinoin in utero (Vitmin A teratology). A consortium agreement has been planned to continue studies of lipid expression and biosynthesis (in culture) in embryonic, fetal and premature skin.