PEROXIDASE AND EOSINOPHILS IN UTERINE ESTROGEN ACTION

这项研究的长期目标是加深对 雌激素在正常和肿瘤组织中作用的分子机制 of the reproductive tract. This research is to focus on the second binding 雌二醇的位点。 该 II 型结合位点受雌激素调节 响应这种类固醇而增殖的组织。 近期目标 这项研究的目的是检验这些 II 型的来源和性质 结合位点。 给大鼠施用雌二醇会导致特定的、 子宫内过氧化物酶含量的雌激素依赖性增加。 这种酶通过浸润的嗜酸性粒细胞转运到子宫中。 它 在该申请中提出，嗜酸性粒细胞也是 子宫 II 型结合位点，并且进一步这些结合位点是 the peroxidase enzyme found in the eosinophils. 因此，嗜酸性粒细胞 将被分离、纯化并分析两个 II 型结合位点 和过氧化物酶活性。 The subcellular localization of each activity 将进行检查以及嗜酸性粒细胞的亚组织定位， II 型结合位点和子宫内的过氧化物酶。 荷尔蒙 还将审查所有这三个方面的监管，以证明 similarities or differences. In addition to the correlative studies a 过氧化物酶活性和 II 型结合位点的直接比较 被执行。 纯化的过氧化物酶结合雌二醇的能力 当在 II 型测定中进行测定时，将有助于确定这两种活性： being associated with one protein. This assay will also be used to study II 型结合和过氧化物酶的各种竞争者和抑制剂 活动。 这些嗜酸性粒细胞蛋白或其他蛋白的可能作用 嗜酸性粒细胞因子将使用雌激素反应性人类进行检查 endometrial carcinoma cell line. These cells will also be used to study II型内源性配体在细胞控制中的作用 分裂和细胞死亡。 These studies may begin to formulate a role of 正常和正常情况下，雌激素介导的生长反应中的嗜酸性粒细胞 肿瘤组织。

The long term objective of this research is to further the understanding of the molecular mechanism of estrogen action in normal and neoplastic tissue of the reproductive tract. This research is to focus on the second binding site for estradiol. This type II binding site is regulated by estrogens in tissue which proliferate in response to this steroid. The immediate goal of this research is to examine the source and nature of these type II binding sites. Estradiol administration to rats results in a specific, estrogen dependent increase in the uterine content of a peroxidase enzyme. This enzyme is transported into the uterus by infiltrating eosinophils. It is proposed in this application that the eosinophils are also the source of the uterine type II binding sites and further that these binding sites are the peroxidase enzyme found in the eosinophils. Therefore, eosinophils will be isolated and purified and assayed for both type II binding sites and peroxidase activity. The subcellular localization of each activity will be examined as well as the sub-tissue localization of the eosinophils, type II binding sites and the peroxidase in the uterus. The hormonal regulation of all three will also be examined in order to demonstrate similarities or differences. In addition to the correlative studies a direct comparison of the peroxidase activity and type II binding sites will be performed. The ability of the purified peroxidase to bind estradiol when assayed in the type II assay will help establish the two activities as being associated with one protein. This assay will also be used to study various competitors and inhibitors of type II binding and peroxidase activity. The possible role of these eosinophil proteins or other eosinophil factors will be examined using an estrogen responsive human endometrial carcinoma cell line. These cells will also be used to study the role of the endogenous ligand for type II in the control of cell division and cell death. These studies may begin to formulate a role of the eosinophils in the estrogen mediated growth response in both normal and neoplastic tissues.