SYNAPTOLOGY OF NEURONS INVOLVED IN REPRODUCTION

我们的目标是确定脑出血的解剖学和神经化学基础 促性腺激素分泌的神经元控制。的“高级加油器” 促性腺激素分泌是促性腺激素释放激素 (GnRH)由一组离散的下丘脑神经元产生。馈送- 雌激素对GnRH释放的反作用部分是通过 控制雌激素活性的雌激素敏感神经元回路 产生促性腺激素释放激素的神经元。GnRH递送系统的控制是 啮齿动物和灵长类动物的组织方式不同，至少部分原因是 解剖学上的差异，如两者的核内位置 促性腺激素释放激素及其相关的雌激素敏感神经元 灵长类动物，而不是大鼠的核间促性腺激素释放系统 其中GnRH神经元位于视前内侧区域。 拟议的实验将有助于实现以下目标：a) 完成雌激素一级环路的鉴定 支持大鼠促性腺激素释放激素神经元的敏感神经元，b)追求 这些环路起源于下丘脑和下丘脑外 面积，c)确定核间的相似程度 大鼠促性腺激素释放激素调控系统和大鼠核内调控 猴子。 电子显微镜双重免疫染色结合变性， 将采用顺行和逆行示踪技术来回答 以下是一些具体问题：1)颈椎的解剖学基础是什么？ 下丘脑内侧基底核GABA~-、多巴胺~-和 大鼠体内的阿片皮质素系统？2)两者之间的相互关系是什么？ 大鼠内侧的GABA能、儿茶酚胺能和GnRH能神经元 视前区？3)下丘脑内侧基底区阿片肽- 含神经元调节去甲肾上腺素/肾上腺素细胞投射 至下丘脑内侧基底内侧和视前内侧Ara 猕猴促性腺激素释放激素能神经元的儿茶酚胺能神经支配 与老鼠体内的情况相似吗？猴促性腺激素释放激素神经元是否直接接受 下丘脑GABA能孕激素受体的信息 神经元？

Our goal is to determine the anatomical and neurochemical bases of neuronal control of gonadotrophin secretion. The "premium movens" of gonadotrophin secretion is the gonadotrophin hormone releasing hormone (GNRH) produced by a discrete group of hypothalamic neurons. The feed- back effects of estrogens of GNRH release are mediated in part by estrogen sensitive neuronal circuits which control the activity of the GNRH producing neurons. The control of GNRH delivery systems is differently organized in rodent and primates, at least in part because of anatomical differences such as the intranuclear location of both the GNRH producing - and their related estrogen sensitive neurons in primates, as opposed to the internuclear GNRH delivery system in rats where the GNRH neurons are located in the medial preoptic area. The proposed experiments will contribute to the following aims: a) to complete the identification of the primary circuits of estrogen sensitive neurons supporting the GNRH neurons in the rat, b) to pursue the origins of these circuits in the hypothalamus and extrahypothalamic areas, c) to determine the degree of similarity between the internuclear system of GNRH control in the rat and the intranuclear control in the monkey. Electron microscopic double immunostaining combined with degeneration, antero- and retrograde tracer techniques will be employed to answer the following specific questions: 1) What is the anatomical basis of the interactions between the mediobasal hypothalamus GABA-,dopamine-, and opiocortin systems in the rat? 2) What is the interrelationship between GABAergic-, catecholaminergic-, and GNRH-neurons in the rat medial preoptic area? 3) How do mediobasal hypothalamus opioid peptide- containing neurons regulate norepinephrine/epinephrine cells projecting to the mediobasal hypothalamus and medial preoptic ara? 4) Is the catecholaminergic innervation of GNRH neurons in the rhesus monkey analogous to that in the rat? Do monkey GNRH neurons receive direct information from GABAergic progesterone receptor-containing hypothalamic neurons?