SYNAPTOLOGY OF NEURONS INVOLVED IN REPRODUCTION

我们的目标是确定神经系统的解剖学和神经化学基础， 促性腺激素分泌的神经元控制。 “优质运动”的 促性腺激素分泌是促性腺激素释放激素 （GNRH）由下丘脑神经元的离散组产生。 饲料- 促性腺激素释放激素释放激素的反作用部分由 雌激素敏感的神经元回路，控制活动的 促性腺激素释放激素神经元。 GNRH输送系统的控制是 在啮齿动物和灵长类动物中的组织方式不同，至少部分原因是 解剖学上的差异，如核内的位置， 垂体促性腺激素释放激素及其相关的雌激素敏感神经元 灵长类动物，而不是大鼠的核间GNRH递送系统 其中GNRH神经元位于内侧视前区。 拟议的实验将有助于实现以下目标： 完成雌激素初级回路的鉴定 大鼠中支持GNRH神经元的敏感神经元，B）追踪 这些回路起源于下丘脑和下丘脑外 c）确定核间的相似程度 大鼠的GNRH控制系统和大鼠的核内控制系统 猴子. 电镜双重免疫染色结合变性， 逆行和逆行示踪技术将被用来回答 以下具体问题：1）什么是解剖学基础， 下丘脑中基底层GABA-、多巴胺-和 大鼠的阿片皮质素系统 2)什么是相互关系， 大鼠内侧核GABA能、儿茶酚胺能和GNRH能神经元 视前区？ 3)下丘脑内侧基底部阿片肽- 含神经元调节去甲肾上腺素/肾上腺素细胞投射 内侧基底下丘脑和内侧视前区 4)是 恒河猴GNRH神经元的儿茶酚胺能神经支配 类似于老鼠的情况吗 猴子的GNRH神经元直接接受 含有GABA能孕酮受体下丘脑的信息 神经元？

Our goal is to determine the anatomical and neurochemical bases of neuronal control of gonadotrophin secretion. The "premium movens" of gonadotrophin secretion is the gonadotrophin hormone releasing hormone (GNRH) produced by a discrete group of hypothalamic neurons. The feed- back effects of estrogens of GNRH release are mediated in part by estrogen sensitive neuronal circuits which control the activity of the GNRH producing neurons. The control of GNRH delivery systems is differently organized in rodent and primates, at least in part because of anatomical differences such as the intranuclear location of both the GNRH producing - and their related estrogen sensitive neurons in primates, as opposed to the internuclear GNRH delivery system in rats where the GNRH neurons are located in the medial preoptic area. The proposed experiments will contribute to the following aims: a) to complete the identification of the primary circuits of estrogen sensitive neurons supporting the GNRH neurons in the rat, b) to pursue the origins of these circuits in the hypothalamus and extrahypothalamic areas, c) to determine the degree of similarity between the internuclear system of GNRH control in the rat and the intranuclear control in the monkey. Electron microscopic double immunostaining combined with degeneration, antero- and retrograde tracer techniques will be employed to answer the following specific questions: 1) What is the anatomical basis of the interactions between the mediobasal hypothalamus GABA-,dopamine-, and opiocortin systems in the rat? 2) What is the interrelationship between GABAergic-, catecholaminergic-, and GNRH-neurons in the rat medial preoptic area? 3) How do mediobasal hypothalamus opioid peptide- containing neurons regulate norepinephrine/epinephrine cells projecting to the mediobasal hypothalamus and medial preoptic ara? 4) Is the catecholaminergic innervation of GNRH neurons in the rhesus monkey analogous to that in the rat? Do monkey GNRH neurons receive direct information from GABAergic progesterone receptor-containing hypothalamic neurons?