STRUCTURAL STUDIES OF RECEPTORS FOR LACTOGENIC HORMONES

在怀孕期间，催乳素和催乳素 胎盘催乳素参与调节 如乳腺分化、类固醇生成、体细胞 多种动物的生长和中间代谢。 对这些过程的监管是通过 催乳素的水平。我们知道产乳素 激素在结构上相似，具有重叠性。 生物活性，但对它们的了解很少 感受器。目前尚不清楚这些荷尔蒙是否会发出 通过每种荷尔蒙特有的受体传递生化信号， 或者，它们是否通过共同的受体发挥作用。 由于催乳激素影响多种生物 过程中，了解受体是很重要的 每一种特定的催乳素在怀孕过程中。鼠标 将被用作研究产乳素受体的生物模型 在怀孕过程中。 将从小鼠乳腺中分离出一种催乳素受体 腺体。纯化的受体将通过结构分析 肽图谱，氨基酸分析，N-末端分析，以及 其他生化手段。将会产生一种抗血清 纯化的催乳素受体及其作为免疫化学物质的应用 探测器。将放射性标记的催乳素与受体交联后 从怀孕不同天取出的器官中，荷尔蒙- 受体复合体将通过二维分析 聚丙烯酰胺凝胶电泳法、免疫印迹法和 放射自显影术用于测定乳头瘤的多重性 产乳素受体。电免疫化学技术将是 用于确定它们之间存在的结构相似性 不同靶器官的催乳素受体。的个体发生学 孕期母体和母体的产乳性受体 胎儿将通过电泳法和免疫染色法进行监测 来自几个组织的受体。病毒的效力和专一性 糖基化催乳素与受体的相互作用将是 使用放射受体分析，交联法和 免疫化学方法学。 这些研究应该提供信息，以改善我们的 对乳源激素受体的认识 小鼠，可能会导致对受体的理解 用于人类催乳素。

During pregnancy, the lactogenic hormones prolactin and placental lactogen participate in the regulation of processes such as mammary gland differentiation, steroidogenesis, somatic growth and intermediary metabolism in a variety of animals. Regulation of these processes is achieved through fluctuations in the levels of the lactogenic hormones. We know that lactogenic hormones are structurally similar and possess overlapping biological activities, but very little is known about their receptors. It is not known whether these hormones send a biochemical signal through a receptor unique to each hormone or, alternatively, whether they function through a common receptor. Since the lactogenic hormones influence a variety of biological processes, it is important to have knowledge about receptors for each specific lactogen over the course of pregnancy. The mouse will be used as a biological model to study lactogenic receptors over the course of gestation. A prolactin receptor will be isolated from mouse mammary glands. The purified receptor will be structurally analyzed by peptide mapping, amino acid analysis, N-terminal analysis, and other biochemical means. An antiserum will be generated with purified prolactin receptor and employed as an immunochemical probe. After crosslinking radiolabelled lactogens to receptors from organs removed on different days of gestation, hormone- receptor complexes will be analyzed by two-dimensional polyacrylamide gel electrophoresis, immunoblotting and autoradiography for determination of the multiplicity of lactogenic receptors. Electroimmunochemical techniques will be used to ascertain structural similarities that exist among prolactin receptors of different target organs. Ontogeny of lactogenic receptors during pregnancy in both the mother and fetus will be monitored by electrophoresis and immunostaining of receptors from several tissues. The potency and specificity of the interaction of glycosylated prolactin with receptors will be examined using radioreceptorassays, crosslinking and immunochemical methodologies. These studies should provide information that will improve our understanding about receptors for lactogenic hormones in the mouse, and perhaps will lead to an understanding about receptors for human lactogenic hormones.