PREMATURE MATERNAL MILK-THYROID LINK TO PRETERM INFANTS
PREMATURE MATERNAL MILK-THYROID LINK TO PRETERM INFANTS
批准号:
3320001
负责人:
LINDA V OBERKOTTER
金额:
$6.46万
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-09-01 至 1988-08-31
关键词:
blood chemistry child physical development developmental nutrition dietary supplements electrofocusing gastrointestinal absorption /transport gel filtration chromatography gestational age high performance liquid chromatography hormone analog hormone regulation /control mechanism human milk human subject iodine lactation newborn animals nutrition related tag premature infant human radioimmunoassay respiratory distress syndrome of newborn thyroid hormones thyrotropin
中文摘要
据我们所知,早产儿的预后已经稳步改善
英文摘要
The prognosis for premature infants has steadily improved as our knowledge
of their metabolic requirements increases. The nutritional needs of these
infants are distinct from those of their full-term counterparts. Likewise,
endocrine regulation is unique, as evidenced by "immature" feedback
regulation of TSH by thyroid hormone (TH) in preterm neonates. A large
body of data indicate the advantages of premature maternal milk (PMM) for
the preterm infant, and the rate of breast feeding among these mothers has
risen dramatically. Analysis of the composition of PMM and how it affects
the neonate is thus mandatory to successful management of these infants.
Our studies and others have confirmed the presence of TH in term breast
milk. Elevated TH levels in breast-fed full- and pre-term infants have
been reported. In order to define the thyroidal components of PMM and
further, evaluate their influence upon the immature thyroid economy of the
preterm infant, we propose to: (1) Isolate, identify, and quantify: (a)
T3 and T4 in PMM at 0, 2, and 4 wks lactation using high performance liquid
chromatography (HPLC) and radioimmunoassay (RIA); and (b) thyroid hormone
analogues and thyromimetic amino acids in PMM by HPLC coupled with
post-column derivatization, in light of their potential bioactivity. (2)
Quantify levels of TSH in PMM by ConA-Sepharose chromatography and TSH-RIA;
and characterize thyroxine-binding capacity by (i) RIA, (ii) isoelectric
focusing and electrophoresis for physical characterization; and (iii)
equilibrium dialysis to determine binding properties. (3) Determine how
thyroid status of PMM-fed infants differs from that of formula-fed preterm
neonates, by assessing serum T4, T3, rT3, TSH, and TBG concentrations in
light of maternal serum and milk levels. (4) Investigate further
absorption by the immature gut of peroral TSH, examining: (a) effect of
gut maturation upon T3 and T4 responses to thyrotropin stimulation in 7-15
day old rat pups; (b) dose-responsiveness of the suckling rat thyroid; and
(c) gut-induced alterations of bio- and/or immuno-reactivity of circulating
thyrotropin resulting from peroral ingestion of TSH, by Sephadex
chromatography, rTSH-RIA, and bioassay of adenylate cyclase activity. The
proposed studies encompass the fields of neonatology, endocrinology,
biochemistry, gastroenterology and physiology. The data generated by these
investigations will lead to a greater understanding of the extent to which
the changing hormonal requirements of the preterm infant may be satisfied
by the dynamic changes in breast milk composition during the course of
lactation.
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MATERNAL IODINE DEFICIENCY--LACTATION AND INFANT GROWTH
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批准号:2200220
-
项目类别:
-
资助金额:$11.89万
-
财政年份:1990
-
负责人:LINDA V OBERKOTTER
-
依托单位:
MATERNAL IODINE DEFICIENCY--LACTATION AND INFANT GROWTH
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批准号:3328629
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项目类别:
-
资助金额:$1.25万
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财政年份:1990
-
负责人:LINDA V OBERKOTTER
-
依托单位:
MATERNAL IODINE DEFICIENCY--LACTATION AND INFANT GROWTH
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批准号:3328628
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项目类别:
-
资助金额:$12.49万
-
财政年份:1990
-
负责人:LINDA V OBERKOTTER
-
依托单位:
MATERNAL IODINE DEFICIENCY--LACTATION AND INFANT GROWTH
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批准号:3328630
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项目类别:
-
资助金额:$11.44万
-
财政年份:1990
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负责人:LINDA V OBERKOTTER
-
依托单位:
SMALL INSTRUMENTATION PROGRAM
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批准号:3525102
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项目类别:
-
资助金额:$0.54万
-
财政年份:1989
-
负责人:LINDA V OBERKOTTER
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
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批准号:3512820
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项目类别:
-
资助金额:$0.6万
-
财政年份:1988
-
负责人:LINDA V OBERKOTTER
-
依托单位:
SMALL INSTRUMENTATION PROGRAM
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批准号:3525090
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项目类别:
-
资助金额:$0.5万
-
财政年份:1988
-
负责人:LINDA V OBERKOTTER
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
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批准号:3512818
-
项目类别:
-
资助金额:$0.45万
-
财政年份:1988
-
负责人:LINDA V OBERKOTTER
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
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批准号:3512819
-
项目类别:
-
资助金额:$0.45万
-
财政年份:1988
-
负责人:LINDA V OBERKOTTER
-
依托单位:
PREMATURE MATERNAL MILK--THYROID LINK TO PRETERN INFANT
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批准号:3320000
-
项目类别:
-
资助金额:$6.58万
-
财政年份:1985
-
负责人:LINDA V OBERKOTTER
-
依托单位:
PREMATURE MATERNAL MILK-THYROID LINK TO PRETERM INFANTS
-
批准号:3320002
-
项目类别:
-
资助金额:$6.27万
-
财政年份:1985
-
负责人:LINDA V OBERKOTTER
-
依托单位: