CELL INTERACTIONS AND THE DEVELOPMENT OF SKELETAL MUSCLE

细胞相互作用和骨骼肌的发育

• 批准号: 3319091
• 负责人: MICHAEL T CROW
• 金额: $ 10.96万
• 依托单位: UNIVERSITY OF HOUSTON
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-08-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3319091
• 关键词: bungarotoxins; cartilage development; cell cell interaction; cell differentiation; cell type; chondrocytes; electrostimulus; enzyme linked immunosorbent assay; extracellular matrix; fibroblasts; gel electrophoresis; histogenesis; innervation; limbs; mixed tissue /cell culture; monoclonal antibody; motor neurons; muscle cells; mutant; myoblasts; myofibrils; myogenesis; neuromuscular blocking agents; neuromuscular function; striated muscles; tetrodotoxin; tissue /cell culture; tubocurarine

The normal development of the vertebrate limb is dependent on the establishment of appropriate interactions among various cell and tissue types. One of the major components of the limb is skeletal muscle. The "blueprint" or plan specifying the precise arrangement and differentiation of the fiber types of skeletal muscle is established early in development and in close temporal association with innervation of the limb and the organizing influence of the connective tissue. The studies proposed here focus on the role of that these interactions play in establishing and maintaining the early pattern of muscle fiber types and controlling the subsequent development of the muscle fibers within the context of this "blueprint". These questions will be addressed in both the developing chicken and rat hindlimbs using a combination of embryo manipulations, in vitro culture procedures, and immunochemical analyses. In vivo, the expression of fiber type- and developmental stage-specific isoforms will be analyzed in limbs in which the normal interactions between the connective tissue and muscle and nerve and muscle has been altered or disrupted. In culture, the development and self-specializing capacity of muscle cells in a) the complete absence of interaction with other cell types, and b) in coculture with motorneurons and muscle "fibroblasts" will be examined. Preliminary data suggest that such interactions may affect expression by making explicit differences which are not normally apparent in culture. The identification of cell interactions that regulate and control the expression of muscle cell types and the reconstruction of these interactions in culture would be both an important conceptual and methodological advance in understanding the cellular mechanisms controlling gene expression and muscle fiber diversification during development.

脊椎动物肢体的正常发育取决于 在各种细胞和组织之间建立适当的相互作用 类型。 肢体的主要组成部分之一是骨骼肌。 这 “蓝图”或计划指定精确的布置和差异化 骨骼肌的纤维类型是在发育早期建立的 并与肢体的神经支配紧密相关 结缔组织的组织影响。 这里提出的研究 专注于这些互动在建立和 保持肌肉纤维类型的早期模式并控制 随后在此背景下开发肌肉纤维 “蓝图”。 这些问题都将在开发中解决 鸡肉和老鼠后肢，结合了胚胎操纵， 体外培养程序和免疫化学分析。 体内 纤维类型和发育阶段特异性同工型的表达将是 在肢体中分析，结缔组织之间的正常相互作用 组织，肌肉，神经和肌肉已改变或破坏。 在 文化，肌肉细胞的发展和自我专业能力 a）与其他细胞类型完全没有相互作用，b） 将检查与运动神经元和肌肉“成纤维细胞”的共培养。 初步数据表明，这种相互作用可能会影响表达 使通常在文化中显而易见的明确差异。 鉴定调节和控制的细胞相互作用 肌肉细胞类型的表达和这些的重建 文化中的互动既是重要的概念，又是 理解控制的细胞机制方面的方法学进步 发育过程中的基因表达和肌肉纤维多样化。