Core--Molecular resources

核心--分子资源

• 批准号: 7347549
• 负责人: MICHAEL T CROW
• 金额: $ 27.1万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7347549
• 关键词: Adenoviruses; Arts; Asthma; Cells; Chimeric Proteins; Communication; Crows; Cytoskeletal Modeling; Cytoskeletal Proteins; Electronic Mail; Evaluation; Facility Construction Funding Category; Feedback; Floor; Future; Glutathione S-Transferase; Housing; Human Resources; Hypersensitivity; Image; Laboratories; Medicine; Methods; Molecular; Mutagenesis; Principal Investigator; Production; Protein Chemistry; Purpose; Quality Control; Reagent; Recombinants; Research; Research Personnel; Resources; Retroviridae; Screening procedure; Services; Site; Specialist; Speed; System; Time; Two-Hybrid System Techniques; Yeasts; base; cost; programs; protein protein interaction; research study; respiratory; vector; yeast two hybrid system

The (Molecular Resources) core, is a newly established core, which will exist within the Center for Translational Respiratory Medicine located on the fifth floor of the Johns Hopkins Asthma and Allergy Building. This Core will directed by Dr. Michael T. Crow, who along with his capable co-investigators and skilled technicians, will assure strict quality control and continuity among the various molecular reagents shared by the various projects. The Core will provide sophisticated, state-of-the-art molecular methods and reagents which will facilitate the characterization and evaluation of key components associated with cytoskeletal organization, cytoskeletal protein-protein interactions, and imaging of cytoskeletal components within the cell. It will be fully utilized by all Projects in this PPG competitive renewal. Specific services to be provided are dictated by the Specific Aims of the Projects and include recombinant adenovirus and retrovirus production, site-directed and chimeric mutagenesis of key cytoskeletal regulatory molecules, the production of GST- and HIS-tagged fusion proteins for detecting and characterizing protein-protein interactions. In addition, the Core provides the necessary expertise, reagents, and controls for yeast two-hybrid screening and mammalian two-hybrid assays. It will be housed in the laboratories of Drs. Crow and Wadgaonkar, which are contiguous with Dr Garcia's laboratory and in close proximity to other investigators, insuring constant feedback between the Core and PPG investigators. In addition, the Core Leader and his staff will meet monthly with PPG leaders to discuss and establish priorities for current and future needs for reagents.

(分子资源)核心，是一个新建立的核心，将存在于位于约翰·霍普金斯哮喘和过敏大楼五楼的转化呼吸医学中心。这一核心将由迈克尔·T·克罗博士指导，他与他能干的合作研究人员和熟练的技术人员将确保严格的质量控制和不同项目共享的各种分子试剂之间的连续性。核心将提供复杂的、最先进的分子方法和试剂，这将促进与细胞骨架组织、细胞骨架蛋白质-蛋白质相互作用相关的关键成分的表征和评估，以及细胞内细胞骨架成分的成像。在这次PPG竞争续签中，它将被所有项目充分利用。将提供的特定服务 所提供的是由项目的具体目标决定的，包括重组腺病毒和逆转录病毒的生产，关键细胞骨架调节分子的定点和嵌合突变，用于检测和表征蛋白质-蛋白质相互作用的GST和His标记的融合蛋白的生产。此外，Core还为酵母双杂交筛选和哺乳动物双杂交分析提供必要的专业知识、试剂和控制。它将被安置在克罗博士和瓦德冈卡博士的实验室里，这两个实验室与加西亚博士的实验室相邻，离其他研究人员很近，以确保 核心调查人员和PPG调查人员之间的持续反馈。此外，核心领导和他的工作人员将每月与PPG领导人会面，讨论并确定试剂当前和未来需求的优先事项。