MICROHETEROGENEITY OF HUMAN ALPHA-FETOPROTEIN
人类 α-胎蛋白的微观异质性
基本信息
- 批准号:3322035
- 负责人:
- 金额:$ 13.36万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1987
- 资助国家:美国
- 起止时间:1987-09-01 至 1995-01-31
- 项目状态:已结题
- 来源:
- 关键词:Macaca mulatta affinity chromatography alpha fetoprotein autoradiography biological polymorphism chemical structure function embryo /fetus embryo /fetus cell /tissue epidermal growth factor gel filtration chromatography gestational age insulinlike growth factor laboratory rat lectin mammalian embryology nucleic acid hybridization nucleic acid probes pregnancy protein biosynthesis protein structure radioimmunoassay southern blotting tissue /cell culture
项目摘要
Alpha fetoprotein (AFP) is a glycoprotein which is produced in high
amounts during pregnancy. Biochemically, AFP exhibits charge lectin
microheterogeneity. Results from our laboratory have shown that the
microheterogeneous nature of AFP changes markedly during normal
development. However, it is currently not certain if these findings
reflect a physiological change in AFP function or merely indicate an
alteration in the biochemistry of this glycoprotein.
AFP isolated from term core blood, although not mitogenic itself, can
enhance the mitogenic activity of growth factors and may function to
modulate cell proliferation during fetal development. No mitogenic
activity was observed in midgestation amniotic fluid, which also
contains increased levels of AFP. Thus, AFP proliferative activity may
vary with fetal development. The relationship between developmental
changes in AFP microheterogeneity and biological activity is of major
interest.
In the series of experiments proposed herein, we will attempt to
characterize the developmental aspects of AFP microheterogeneity and
biological activity. We will use the monkey as a model to carefully
define these ontogenic changes and to compliment and extend data
obtained from the use of human fetal material, which is obviously
limited. The charge and lectin microheterogeneity of AFP will be
evaluated in the species throughout gestation. Monkey fetal tissue will
be removed at various stages of gestation to allow the characterization
of the production of AFP by these tissues. Additionally, we will use
both the monkey and the human to elucidate the relationships between
charge and lectin microheterogeneity and the expression of AFP
proliferative activity. Finally, the biochemical basis for AFP
microheterogeneity and biological activity will be elucidated. The
results of these studies will greatly add to our knowledge of the
biochemical and physiological importance of AFP to normal fetal
development.
甲胎蛋白(AFP)是一种高表达的糖蛋白。
在怀孕期间的数量。AFP在生物化学上显示电荷凝集素
微观异质性。我们实验室的结果表明,
甲胎蛋白的微异质性在正常状态下发生显著变化
发展。然而,目前还不能确定这些发现是否
反映AFP功能的生理变化或仅仅表明
这种糖蛋白的生物化学变化。
从足月核心血中分离出的甲胎蛋白,虽然本身不是有丝分裂原,但可以
增强生长因子的有丝分裂活性,并可能起到
在胎儿发育过程中调节细胞增殖。没有丝裂原
在妊娠中期的羊水中观察到活性,这也是
含有升高的甲胎蛋白水平。因此,AFP的增殖活性可能
随胎儿发育而变化。发展与发展之间的关系
甲胎蛋白微观异质性和生物活性的变化是主要的
利息。
在这里提出的一系列实验中,我们将尝试
描述甲胎蛋白微异质性的发育特征和
生物活性。我们将以猴子为模型,小心地
定义这些个体发生的变化并补充和扩展数据
从使用人类胎儿的材料中获得,这显然是
有限的。AFP的电荷和凝集素的微观不均一性将是
在整个怀孕期间在物种中进行评估。猴子的胎儿组织会
在怀孕的不同阶段被移除,以允许表征
这些组织产生甲胎蛋白的能力。此外,我们将使用
无论是猴子还是人,都要阐明它们之间的关系
电荷和凝集素的微观异质性与甲胎蛋白的表达
增殖活性。最后,甲胎蛋白的生化基础
微生物的异质性和生物活性将被阐明。这个
这些研究的结果将极大地增加我们对
甲胎蛋白对正常胎儿的生化和生理意义
发展。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
BROOKS ALLEN KEEL其他文献
BROOKS ALLEN KEEL的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('BROOKS ALLEN KEEL', 18)}}的其他基金
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
少数民族高中生研究学徒计划
- 批准号:
3512961 - 财政年份:1990
- 资助金额:
$ 13.36万 - 项目类别:
相似海外基金
Cellular membrane affinity chromatography kit for drug discovery
用于药物发现的细胞膜亲和层析试剂盒
- 批准号:
10506915 - 财政年份:2021
- 资助金额:
$ 13.36万 - 项目类别:
Cellular membrane affinity chromatography kit for drug discovery
用于药物发现的细胞膜亲和层析试剂盒
- 批准号:
10325006 - 财政年份:2021
- 资助金额:
$ 13.36万 - 项目类别:
SBIR Phase I: A New Class of Immobilized Metal Affinity Chromatography Resins
SBIR 第一阶段:一类新型固定金属亲和色谱树脂
- 批准号:
1746198 - 财政年份:2018
- 资助金额:
$ 13.36万 - 项目类别:
Standard Grant
Marine speciation of nickel using immobilized nickel affinity chromatography
使用固定镍亲和色谱法测定镍的海洋形态
- 批准号:
512537-2017 - 财政年份:2017
- 资助金额:
$ 13.36万 - 项目类别:
University Undergraduate Student Research Awards
I-Corps: Commercialization of Immobilized Metal Affinity Chromatography Resins Based on Nanomaterials
I-Corps:基于纳米材料的固定化金属亲和层析树脂的商业化
- 批准号:
1404605 - 财政年份:2014
- 资助金额:
$ 13.36万 - 项目类别:
Standard Grant
Antibody Purification via Affinity Chromatography that Utilizes the Unconventional Nucleotide Binding Site
利用非常规核苷酸结合位点通过亲和色谱法纯化抗体
- 批准号:
1263713 - 财政年份:2013
- 资助金额:
$ 13.36万 - 项目类别:
Continuing Grant
Development of multivalent DNA network based affinity chromatography diagnostics for isolating circulating tumour cells
开发基于多价 DNA 网络的亲和色谱诊断法,用于分离循环肿瘤细胞
- 批准号:
425749-2012 - 财政年份:2012
- 资助金额:
$ 13.36万 - 项目类别:
Postgraduate Scholarships - Master's
Next-Generation Affinity Chromatography with PEGylated Ligands
使用聚乙二醇化配体的新一代亲和色谱法
- 批准号:
1159886 - 财政年份:2012
- 资助金额:
$ 13.36万 - 项目类别:
Standard Grant
Immobilized zirconium ion affinity chromatography for specific enrichment of phosphoproteins
用于磷蛋白特异性富集的固定化锆离子亲和层析
- 批准号:
19560760 - 财政年份:2007
- 资助金额:
$ 13.36万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Accelerating drug discovery using frontal affinity chromatography/mass spectrometry
使用正面亲和色谱/质谱加速药物发现
- 批准号:
234753-2000 - 财政年份:2003
- 资助金额:
$ 13.36万 - 项目类别:
Collaborative Research and Development Grants