TERATOCARCINOMA CELLS--CELL ADHESION AND DIFFERENTIATION
畸胎癌细胞——细胞粘附和分化
基本信息
- 批准号:3328956
- 负责人:
- 金额:$ 10.39万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1990
- 资助国家:美国
- 起止时间:1990-09-01 至 1995-08-31
- 项目状态:已结题
- 来源:
- 关键词:cell adhesion cell adhesion molecules cell cell interaction cell differentiation cytoskeletal proteins extracellular matrix gene expression gene mutation immunofluorescence technique interference microscopy molecular cloning molecular genetics neoplastic cell nucleic acid probes receptor binding teratoma tissue /cell culture video recording system
项目摘要
In recent years a number of adhesion and cytoskeletal molecules have been
isolated and characterized at the molecular level. The identification of
those molecules has provided a basis for a molecular understanding of the
process of cell adhesion. The challenge now is to learn how these
molecules are utilized for cell-cell and cell-substrate adhesions.
This project examines two aspects of the adhesion phenomenon. First,
methods have been developed for examining cell adhesion as a sequence of
discrete events. One goal will be to characterize those events using known
molecular probes. The events include initial binding with the substrate,
collection of adhesion molecules at the site of contact, coupling the
adhesion complex to the cytoskeleton, a strengthening of the adhesion over
several orders of magnitude, and finally, the loss of the adhesion.
The F9 teratocarcinoma cell line will be used for this study. F9 cells
have the capacity to differentiate following treatment with retinoic acid.
Prior to retinoic acid treatment the cells are epithloid. As the cells
differentiate they become fibroblastic. Many adhesion molecules are known
in the F9 system. The second aspect of this proposal, therefore, is to
determine what molecular changes enable cells to undergo the predictable
phenotypic conversion from an epithelial to a fibroblastic phenotype. The
studies will be greatly aided by an adhesion mutant that is deficient in a
specific integrin subtype.
Adhesive properties of cells have a dramatic influence on cell behavior.
Thus, tissue construction, morphogenesis, invasiveness and metastasis all
have an adhesive requirement that directs or impinges upon cell behavior.
近年来,一些黏附和细胞骨架分子被
在分子水平上进行分离和鉴定。身份的鉴定
这些分子为从分子水平上理解
细胞黏附的过程。现在的挑战是了解这些
分子被用于细胞与细胞和细胞与底物的粘合。
这个项目考察了粘连现象的两个方面。第一,
已经开发了检查细胞黏附的方法,作为一系列
离散事件。一个目标将是使用已知信息来描述这些事件
分子探测器。这些事件包括与衬底的初始结合,
在接触部位聚集黏附分子,连接
与细胞骨架的黏附复合体,加强了黏附
几个数量级,最后是粘附力的丧失。
这项研究将使用F9畸胎癌细胞系。F9细胞
在维甲酸治疗后有分化能力。
在维甲酸治疗之前,细胞是上皮样的。因为细胞
分化为成纤维细胞。许多黏附分子是已知的
在F9系统中。因此,这项建议的第二个方面是
确定哪些分子变化使细胞能够经历可预测的
表型由上皮型向成纤维细胞表型转化。这个
粘附性突变体的缺失将极大地帮助研究
特定的整合素亚型。
细胞的粘附性对细胞的行为有很大影响。
因此,组织结构、形态发生、侵袭和转移
具有引导或影响细胞行为的粘附性要求。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
DAVID MCCLAY其他文献
DAVID MCCLAY的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('DAVID MCCLAY', 18)}}的其他基金
Transcriptional Casualty in Embryonic Morphogenesis: from the Specifications GRN
胚胎形态发生中的转录伤亡:来自 GRN 规范
- 批准号:
8092700 - 财政年份:2010
- 资助金额:
$ 10.39万 - 项目类别:
Sea Urchin Developmental Biology Conference XIV
第十四届海胆发育生物学会议
- 批准号:
6507470 - 财政年份:2002
- 资助金额:
$ 10.39万 - 项目类别:
相似海外基金
Collaborative Research: NSF-BSF: How cell adhesion molecules control neuronal circuit wiring: Binding affinities, binding availability and sub-cellular localization
合作研究:NSF-BSF:细胞粘附分子如何控制神经元电路布线:结合亲和力、结合可用性和亚细胞定位
- 批准号:
2321481 - 财政年份:2024
- 资助金额:
$ 10.39万 - 项目类别:
Continuing Grant
Collaborative Research: NSF-BSF: How cell adhesion molecules control neuronal circuit wiring: Binding affinities, binding availability and sub-cellular localization
合作研究:NSF-BSF:细胞粘附分子如何控制神经元电路布线:结合亲和力、结合可用性和亚细胞定位
- 批准号:
2321480 - 财政年份:2024
- 资助金额:
$ 10.39万 - 项目类别:
Continuing Grant
Non-Canonical Roles for Cell-Adhesion Molecules in Presynaptic Assembly
细胞粘附分子在突触前组装中的非典型作用
- 批准号:
10751904 - 财政年份:2023
- 资助金额:
$ 10.39万 - 项目类别:
Mechanisms underlying the roles of cell adhesion molecules in the circadian timing system
细胞粘附分子在昼夜节律系统中的作用机制
- 批准号:
RGPIN-2020-05262 - 财政年份:2022
- 资助金额:
$ 10.39万 - 项目类别:
Discovery Grants Program - Individual
Mechanisms underlying the roles of cell adhesion molecules in the circadian timing system
细胞粘附分子在昼夜节律系统中的作用机制
- 批准号:
RGPIN-2020-05262 - 财政年份:2021
- 资助金额:
$ 10.39万 - 项目类别:
Discovery Grants Program - Individual
The role of cadherin cell adhesion molecules in postnatal porcine islet cell function.
钙粘蛋白细胞粘附分子在出生后猪胰岛细胞功能中的作用。
- 批准号:
449549 - 财政年份:2020
- 资助金额:
$ 10.39万 - 项目类别:
Studentship Programs
Role of insect olfactory receptors and cell adhesion molecules in circuit organization
昆虫嗅觉受体和细胞粘附分子在电路组织中的作用
- 批准号:
2006471 - 财政年份:2020
- 资助金额:
$ 10.39万 - 项目类别:
Continuing Grant
Elucidation of epithelial-connective tissue interactions mediated by cell adhesion molecules in drug-induced gingival hyperplasia
阐明药物诱导的牙龈增生中细胞粘附分子介导的上皮-结缔组织相互作用
- 批准号:
20K23026 - 财政年份:2020
- 资助金额:
$ 10.39万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
Development of novel intervening approaches to cancer progression by comprehensive analyses of interactions between cell adhesion molecules
通过综合分析细胞粘附分子之间的相互作用,开发癌症进展的新干预方法
- 批准号:
20K21539 - 财政年份:2020
- 资助金额:
$ 10.39万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Elucidation of the mechanism of gastric cancer progression and development of innovative therapeutic strategies focusing on cell adhesion molecules
阐明胃癌进展机制并开发以细胞粘附分子为重点的创新治疗策略
- 批准号:
20K22831 - 财政年份:2020
- 资助金额:
$ 10.39万 - 项目类别:
Grant-in-Aid for Research Activity Start-up