DEVELOPMENTAL CONTROL OF THE DIAPHRAGM AND UPPER AIRWAYS

这项研究的目的是为了更好地了解 协调上呼吸道肌肉活动的机制 与出生后发育过程中的横隔膜相同。功能障碍 这一系统导致早产儿发生阻塞性呼吸暂停。 患有阻塞性睡眠呼吸暂停的婴儿和成人。这些实验是 设计用来测量膜性能的变化 控制横隔膜和上呼吸道的呼吸运动神经元。 这项建议的具体目的是确定内在的作用 这些运动神经元的特性在它们的呼吸形成中起着作用 发育过程中的放电行为。更具体地说，执行内部操作 运动神经元的特性控制它的神经元行为？这个 控制这一行为的细胞和离子机制的演变 将在出生后的发育过程中进行研究。从这些研究中，我们发现 希望弄清在这个过程中有没有什么不同 这两个机动车池之间的成熟程度，如果是的话，是什么关系 获得其成体放电特征的顺序？ 这些呼吸系统的主动膜和被动膜特性 运动神经元将使用细胞内记录和 贴标签技术。这些性质背后的离子机制 将使用单电极电流和电压钳位在 包括体内和体外的哺乳动物制剂。一种体外脊柱 脊髓和脑干自将被开发，以研究 发育过程中特定离子电流的变化。

The goal of this research is to gain a better understanding of the mechanisms which coordinate the activation of the upper airway muscles with that of the diaphragm during postnatal development. Dysfunction in this system results in episodes of obstructive apneas found in premature infants and adults with obstructive sleep apnea. These experiments are designed to measure the changes in the membrane properties of the respiratory motoneurons controlling the diaphragm and upper airways. The specific aim of this proposal is to determine the role the intrinsic properties of these motoneurons play in shaping their respiratory discharge behavior during development. More specifically, do intrinsic properties of the motoneuron control its neuronal behavior? The evolution of cellular and ionic mechanisms controlling this behavior will be studied during postnatal development. From these studies, we hope to ascertain if there is any difference in the process of maturation between these two motor pools and if so, what is the relative sequence of attaining their adult discharge characteristics? The active and passive membrane properties of these respiratory motoneurons will be investigated using intracellular recording and labeling techniques. The ionic mechanisms underlying these properties will be delineated using single electrode current and voltage clamp in both in vivo and in vitro mammalian preparations. An in vitro spinal cord and brainstem since will be developed in order to study the alteration in specific ionic currents during development.