HIGH RESOLUTION GENETIC MAPPING: STRATEGY-BASED APPROACH

本研究的总体目标是开发一种高 分辨率作图，最初用于染色体17和X上的标记;以及 随后，对于其他染色体。 该资源将由 在60个CEPH中鉴定由交叉定义的减数分裂片段 家庭 减数分裂作图板将大大提高效率 和基因图谱的可靠性。 CEPH的潜在解决方案 常染色体和X染色体的同源性分别为0.01 cM和0.02 cM。在 为了最有效地开发映射面板资源， 需要修改和扩展现有的计算机程序， 目前用于CEPH标记数据的单体型分析和质量控制。 染色体17和X的标记物的电流密度足以 使用这些简单的算法，几乎可以确定所有的交叉点。 每个交叉的位置将通过重复键入 最近的侧翼标记 将筛选额外的减数分裂 紧密连锁的基因座之间的重组体，不能被其他基因排序， 手段 其他标记物的选择性分型将在有指导的 努力缩小每个交叉点周围的差距。 这一战略将 提高分辨率，同时改进 新的标记。 将遗传数据转化为 物理映射表示将被开发为一种简单的方法， 将遗传作图和物理作图的结果相结合， 问题研究

The overall objective of this study is to develop a resource for high resolution mapping, initially for markers on chromosomes 17 and X; and subsequently, for other chromosomes. The resource will be developed by identifying meiotic fragments that are defined by crossovers in 60 CEPH families. The meiotic mapping panel will greatly improve the efficiency and reliability of genetic mapping. The potential resolution of the CEPH resource is about 0.01 cM for autosomes and 0.02 cM for chromosome X. In order to develop the mapping panel resource most efficiently, it will be necessary to modify and extend existing computer programs that are currently used for haplotyping and quality control of CEPH marker data. The current density of markers for chromosomes 17 and X is sufficient to identify nearly all crossovers unambiguously using these simple algorithms. The position of each crossover will be validated by duplicate typing of the nearest flanking markers. Additional meioses will be screened for recombinants between tightly linked loci that cannot be ordered by other means. Selective typing of additional markers will be done in a directed effort to close the gap surrounding each crossover. This strategy will improve resolution and, at the same time, refine the localization of the new marker. Computer algorithms for translating genetic data into a physical mapping representation will be developed as a simple means of combining and comparing the results of genetic and physical mapping studies.